Ha Manh Tuan, Tran Manh Hung, Phuong Thien Thuong, Kim Jeong Ah, Woo Mi Hee, Choi Jae Sue, Lee Suhyun, Lee Jeong Hyung, Lee Hyeong Kyu, Min Byung Sun
College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea; Department of Bioactive Products, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay District, Hanoi, Viet Nam.
Biomedical Science Department, VNUK Institute for Research and Executive Education, The University of Danang, 158A Le Loi, Hai Chau District, Danang City, Viet Nam.
Bioorg Med Chem Lett. 2017 Jul 1;27(13):2946-2952. doi: 10.1016/j.bmcl.2017.05.006. Epub 2017 May 4.
A phytochemical investigation into the bark of Erythrophleum fordii yielded four new compounds, two new cassaine diterpenoids (erythrofordin T and U, 1 and 2) and two new cassaine diterpenoid amines (erythroformine A and B, 6 and 7), as well as nine known compounds. We report for the first time the isolation of erythrofordin V (3) from a natural source and that of the remaining eight known diterpenoids (4-5, 8-13) from E. fordii. All structures were elucidated using spectroscopic analysis. Cytotoxic activity of the isolated compounds (1-13) was examined in vitro against three non-small cell lung cancer cell lines (A549, NCI-H1975, and NCI-H1229) using the MTT assay. Cassaine diterpene amines (6-10, 12, 13) exhibited potent cytotoxic activity against all three cell lines with IC values between 0.4μM and 5.9μM. Erythroformine B (7) significantly induced apoptosis in all three cancer cells in a concentration-dependent manner.
对牛耳枫树皮进行的植物化学研究得到了四种新化合物，两种新的格木烷二萜（牛耳枫素T和U，1和2）以及两种新的格木烷二萜胺（牛耳枫明A和B，6和7），还有九种已知化合物。我们首次从天然来源中分离出牛耳枫素V（3）以及从牛耳枫中分离出其余八种已知二萜（4 - 5，8 - 13）。所有结构均通过光谱分析得以阐明。使用MTT法在体外检测了分离出的化合物（1 - 13）对三种非小细胞肺癌细胞系（A549、NCI - H1975和NCI - H1229）的细胞毒性活性。格木烷二萜胺（6 - 10、12、13）对所有三种细胞系均表现出强效细胞毒性活性，IC值在0.4μM至5.9μM之间。牛耳枫明B（7）以浓度依赖的方式在所有三种癌细胞中显著诱导凋亡。
