Liu Xiaohui, Ouyang Ting, Ouyang Hongsheng, Ren Linzhu
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, China.
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, China.
Virus Res. 2017 Jun 2;237:14-21. doi: 10.1016/j.virusres.2017.05.007. Epub 2017 May 12.
Real-time and visual tracking of viral infection is crucial for elucidating the infectious and pathogenesis mechanisms. To track the virus successfully, an efficient labeling method is necessary. In this review, we first discuss the practical labeling techniques for virus tracking in live cells. We then describe the current knowledge of interactions between RNA viruses (especially influenza viruses, immunodeficiency viruses, and Flaviviruses) and host cellular structures, obtained using single particle labeling techniques combined with real-time fluorescence microscopy. Single particle labeling provides an easy system for understanding the RNA virus life cycle.
实时可视化追踪病毒感染对于阐明感染和发病机制至关重要。为了成功追踪病毒，需要一种有效的标记方法。在本综述中，我们首先讨论活细胞中病毒追踪的实用标记技术。然后，我们描述了使用单颗粒标记技术结合实时荧光显微镜获得的关于RNA病毒（特别是流感病毒、免疫缺陷病毒和黄病毒）与宿主细胞结构之间相互作用的当前知识。单颗粒标记为理解RNA病毒生命周期提供了一个简便的系统。
