Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA.
Trends Biotechnol. 2011 Jul;29(7):307-13. doi: 10.1016/j.tibtech.2011.02.006. Epub 2011 Apr 27.
Traditional methods that rely on viral isolation and culture techniques continue to be the gold standards used for detection of infectious viral particles. However, new techniques that rely on visualization of live cells can shed light on understanding virus-host interaction for early stage detection and potential drug discovery. Live-cell imaging techniques that incorporate fluorescent probes into viral components provide opportunities for understanding mRNA expression, interaction, and virus movement and localization. Other viral replication events inside a host cell can be exploited for non-invasive detection, such as single-virus tracking, which does not inhibit viral infectivity or cellular function. This review highlights some of the recent advances made using these novel approaches for visualization of viral entry and replication in live cells.
传统的方法依赖于病毒分离和培养技术，仍然是用于检测传染性病毒颗粒的金标准。然而，新的技术依赖于活细胞的可视化，可以揭示病毒-宿主相互作用，用于早期检测和潜在的药物发现。将荧光探针整合到病毒成分中的活细胞成像技术为理解 mRNA 表达、相互作用以及病毒运动和定位提供了机会。宿主细胞内的其他病毒复制事件也可以被用于非侵入性检测，例如单病毒跟踪，它不会抑制病毒的感染力或细胞功能。本综述强调了一些使用这些新方法在活细胞中可视化病毒进入和复制的最新进展。
