Jung Hyun Min, Castranova Daniel, Swift Matthew R, Pham Van N, Venero Galanternik Marina, Isogai Sumio, Butler Matthew G, Mulligan Timothy S, Weinstein Brant M
Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Department of Anatomy, School of Medicine, Iwate Medical University, Morioka 020-8505, Japan.
Development. 2017 Jun 1;144(11):2070-2081. doi: 10.1242/dev.145755. Epub 2017 May 15.
The lymphatic vascular system is a hierarchically organized complex network essential for tissue fluid homeostasis, immune trafficking and absorption of dietary fats in the human body. Despite its importance, the assembly of the lymphatic network is still not fully understood. The zebrafish is a powerful model organism that enables study of lymphatic vessel development using high-resolution imaging and sophisticated genetic and experimental manipulation. Although several studies have described early lymphatic development in the fish, lymphatic development at later stages has not been completely elucidated. In this study, we generated a new transgenic zebrafish that uses a () promoter to drive strong EGFP expression in lymphatic vessels at all stages of development and in adult zebrafish. We used this line to describe the assembly of the major vessels of the trunk lymphatic vascular network, including the later-developing collateral cardinal, spinal, superficial lateral and superficial intersegmental lymphatics. Our results show that major trunk lymphatic vessels are conserved in the zebrafish, and provide a thorough and complete description of trunk lymphatic vessel assembly.
淋巴血管系统是一个层次结构组织的复杂网络，对人体组织液稳态、免疫运输和膳食脂肪吸收至关重要。尽管其很重要，但淋巴网络的组装仍未完全了解。斑马鱼是一种强大的模式生物，能够使用高分辨率成像以及复杂的遗传和实验操作来研究淋巴管发育。虽然有几项研究描述了鱼类早期淋巴发育，但后期的淋巴发育尚未完全阐明。在本研究中，我们生成了一种新的转基因斑马鱼，其使用（）启动子在发育的所有阶段以及成年斑马鱼的淋巴管中驱动强EGFP表达。我们使用该品系来描述躯干淋巴血管网络主要血管的组装，包括后期发育的侧副主淋巴管、脊髓淋巴管、外侧浅表淋巴管和节间浅表淋巴管。我们的结果表明，斑马鱼的主要躯干淋巴管是保守的，并提供了对躯干淋巴管组装的全面而完整的描述。
