Yaniv Karina, Isogai Sumio, Castranova Daniel, Dye Louis, Hitomi Jiro, Weinstein Brant M
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, 6B/309, 6 Center Drive, Bethesda, Maryland 20892, USA.
Nat Med. 2006 Jun;12(6):711-6. doi: 10.1038/nm1427. Epub 2006 May 28.
The lymphatic system has become the subject of great interest in recent years because of its important role in normal and pathological processes. Progress in understanding the origins and early development of this system, however, has been hampered by difficulties in observing lymphatic cells in vivo and in performing defined genetic and experimental manipulation of the lymphatic system in currently available model organisms. Here, we show that the optically clear developing zebrafish provides a useful model for imaging and studying lymphatic development, with a lymphatic system that shares many of the morphological, molecular and functional characteristics of the lymphatic vessels found in other vertebrates. Using two-photon time-lapse imaging of transgenic zebrafish, we trace the migration and lineage of individual cells incorporating into the lymphatic endothelium. Our results show lymphatic endothelial cells of the thoracic duct arise from primitive veins through a novel and unexpected pathway.
近年来，淋巴系统因其在正常和病理过程中的重要作用而备受关注。然而，由于在体内观察淋巴细胞以及在现有模式生物中对淋巴系统进行特定的基因和实验操作存在困难，该系统起源和早期发育的研究进展受到了阻碍。在此，我们表明，光学透明的发育中的斑马鱼为成像和研究淋巴发育提供了一个有用的模型，其淋巴系统具有许多与其他脊椎动物淋巴管共有的形态、分子和功能特征。通过对转基因斑马鱼进行双光子延时成像，我们追踪了整合到淋巴管内皮中的单个细胞的迁移和谱系。我们的结果表明，胸导管的淋巴管内皮细胞通过一条新颖且意想不到的途径起源于原始静脉。
