Center for Nano-Bio Measurement, Korea Research Institute of Standards and Science (KRISS), Daejeon, 34113, Republic of Korea.
Department of Chemistry, Chungnam National University, Daejeon, 34134, Republic of Korea.
J Am Soc Mass Spectrom. 2017 Aug;28(8):1729-1732. doi: 10.1007/s13361-017-1682-3. Epub 2017 May 15.
Corn seed tissue sections were prepared by the tape support method using an adhesive tape, and mass spectrometry imaging (MSI) was performed. The effect of heat generated during sample preparation was investigated by time-of-flight secondary mass spectrometry (TOF-SIMS) imaging of corn seed tissue prepared by the tape support and the thaw-mounted methods. Unlike thaw-mounted sample preparation, the tape support method does not cause imaging distortion because of the absence of heat, which can cause migration of the analytes on the sample. By applying the tape-support method, the corn seed tissue was prepared without structural damage and MSI with accurate spatial information of analytes was successfully performed. Graphical Abstract ᅟ.
采用胶粘带支持法制备玉米种子组织切片，并进行质谱成像（MSI）分析。利用飞行时间二次质谱成像（TOF-SIMS）对采用胶粘带支持法和融解安装法制备的玉米种子组织进行分析，研究了样品制备过程中产生的热效应对玉米种子组织的影响。与融解安装法不同，由于不存在热效应，胶粘带支持法不会导致成像失真，而热效应可能会导致样品上分析物的迁移。采用胶粘带支持法，玉米种子组织在制备过程中没有结构损伤，并且成功地进行了具有分析物精确空间信息的 MSI。
