Quanico Jusal, Franck Julien, Wisztorski Maxence, Salzet Michel, Fournier Isabelle
Université de Lille 1, INSERM, U1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), 59000, Lille, France.
Methods Mol Biol. 2017;1598:21-43. doi: 10.1007/978-1-4939-6952-4_2.
Mapping provides a direct means to assess the impact of protein biomarkers and puts into context their relevance in the type of cancer being examined. To this end, mass spectrometry imaging (MSI) was developed to provide the needed spatial information which is missing in traditional liquid-based mass spectrometric proteomics approaches. Aptly described as a "molecular histology" technique, MSI gives an additional dimension in characterizing tumor biopsies, allowing for mapping of hundreds of molecules in a single analysis. A decade of developments focused on improving and standardizing MSI so that the technique can be translated into the clinical setting. This review describes the progress made in addressing the technological development that allows to bridge local protein detection by MSI to its identification and to illustrate its potential in studying various aspects of cancer biomarker discovery.
图谱绘制提供了一种直接手段来评估蛋白质生物标志物的影响，并将它们在正在研究的癌症类型中的相关性置于具体情境中。为此，开发了质谱成像（MSI）技术以提供传统基于液相的质谱蛋白质组学方法所缺失的必要空间信息。MSI被恰当地描述为一种“分子组织学”技术，它为肿瘤活检的特征描述增添了一个维度，能够在单次分析中对数百种分子进行图谱绘制。十年来的发展集中在改进和标准化MSI技术，以便该技术能够转化应用于临床环境。本综述描述了在解决技术发展方面所取得的进展，这些进展使得能够将MSI的局部蛋白质检测与其鉴定联系起来，并阐明其在研究癌症生物标志物发现的各个方面的潜力。
