Toklu Hale Zerrin, Sakarya Yasemin, Tümer Nihal
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, ARB R5-234, 1200 Newell Drive, PO 100267, Gainesville, FL, 32610, USA.
Geriatric Research Education and Clinical Center, Malcolm Randall Veterans Affairs Medical Center, Gainesville, FL, USA.
Methods Mol Biol. 2017;1598:313-325. doi: 10.1007/978-1-4939-6952-4_16.
Endocrine disorders and autonomic dysfunction are common paradigms following traumatic brain injury (TBI). Alterations in the hypothalamus-pituitary-adrenal (HPA) axis following TBI may result in impaired vasopressor response, energy imbalance, fatigue, depression, or neurological disorders. Autonomic dysfunction is a common disorder following TBI. The sympathetic activity markers on HPA axis can be measured by Western blot protein analysis. Tyrosine hydroxylase, dopamine beta hydroxylase are the key enzymes for the synthesis of norepinephrine; and neuropeptide Y (NPY) is the peptide that is co-stored and co-released with norepinephrine. Thus, the present chapter reviews the experimental protocols for Western blot protein analysis for the measurement of biomarkers that indicate sympathetic activity in brain regions (hypothalamus, pituitary, cerebral cortex, and cerebellum) following TBI.
内分泌失调和自主神经功能障碍是创伤性脑损伤（TBI）后的常见情况。TBI后下丘脑-垂体-肾上腺（HPA）轴的改变可能导致血管升压反应受损、能量失衡、疲劳、抑郁或神经紊乱。自主神经功能障碍是TBI后的常见病症。HPA轴上的交感神经活动标志物可通过蛋白质免疫印迹分析来测量。酪氨酸羟化酶、多巴胺β羟化酶是去甲肾上腺素合成的关键酶；神经肽Y（NPY）是与去甲肾上腺素共同储存和共同释放的肽。因此，本章综述了用于蛋白质免疫印迹分析的实验方案，以测量TBI后指示脑区（下丘脑、垂体、大脑皮层和小脑）交感神经活动的生物标志物。
