Escoffre Jean-Michel, Mauroy Chloé, Portet Thomas, Wasungu Luc, Rosazza Chrystelle, Gilbart Yoann, Mallet Laetitia, Bellard Elisabeth, Golzio Muriel, Rols Marie-Pierre, Teissié Justin
CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, 31077, Toulouse, France.
Université de Toulouse UPS, IPBS, 31077, Toulouse, France.
Biophys Rev. 2009 Dec;1(4):177. doi: 10.1007/s12551-009-0022-7. Epub 2009 Nov 17.
Electropulsation is one of the nonviral methods successfully used to deliver genes into living cells in vitro and in vivo. This approach shows promise in the field of gene and cellular therapies. The present review focuses on the processes supporting gene electrotransfer in vitro. In the first part, we will report the events occurring before, during, and after pulse application in the specific field of plasmid DNA electrotransfer at the cell level. A critical discussion of the present theoretical considerations about membrane electropermeabilization and the transient structures involved in the plasmid uptake follows in a second part.
电脉冲是一种成功用于在体外和体内将基因导入活细胞的非病毒方法。这种方法在基因和细胞治疗领域显示出前景。本综述聚焦于支持体外基因电转染的过程。在第一部分，我们将报告在细胞水平上质粒DNA电转染特定领域中脉冲施加之前、期间和之后发生的事件。第二部分将对目前关于膜电穿孔和质粒摄取所涉及的瞬时结构的理论思考进行批判性讨论。
