Su Yingying, Nykanen Marko, Jahn Kristina A, Whan Renee, Cantrill Laurence, Soon Lilian L, Ratinac Kyle R, Braet Filip
Australian Centre for Microscopy and Microanalysis, The University of Sydney, Madsen Building F09, Sydney, NSW, 2006, Australia.
Kids Research Institute, Children's Hospital Westmead, Westmead, Locked Bag 4001, NSW, 2145, Australia.
Biophys Rev. 2010 Aug;2(3):121-135. doi: 10.1007/s12551-010-0035-2. Epub 2010 Jul 28.
To genuinely understand how complex biological structures function, we must integrate knowledge of their dynamic behavior and of their molecular machinery. The combined use of light or laser microscopy and electron microscopy has become increasingly important to our understanding of the structure and function of cells and tissues at the molecular level. Such a combination of two or more different microscopy techniques, preferably with different spatial- and temporal-resolution limits, is often referred to as 'correlative microscopy'. Correlative imaging allows researchers to gain additional novel structure-function information, and such information provides a greater degree of confidence about the structures of interest because observations from one method can be compared to those from the other method(s). This is the strength of correlative (or 'combined') microscopy, especially when it is combined with combinatorial or non-combinatorial labeling approaches. In this topical review, we provide a brief historical perspective of correlative microscopy and an in-depth overview of correlative sample-preparation and imaging methods presently available, including future perspectives on the trend towards integrative microscopy and microanalysis.
要真正理解复杂生物结构的功能，我们必须整合有关其动态行为和分子机制的知识。光学或激光显微镜与电子显微镜的联合使用，对于我们在分子水平上理解细胞和组织的结构与功能变得越来越重要。两种或更多种不同显微镜技术的这种组合，最好具有不同的空间和时间分辨率极限，通常被称为“相关显微镜术”。相关成像使研究人员能够获得额外的新颖结构-功能信息，并且由于可以将一种方法的观察结果与其他方法的观察结果进行比较，此类信息为感兴趣的结构提供了更高的可信度。这就是相关（或“联合”）显微镜术的优势，尤其是当它与组合或非组合标记方法相结合时。在本专题综述中，我们提供了相关显微镜术的简要历史回顾，并深入概述了目前可用的相关样品制备和成像方法，包括对整合显微镜术和微分析趋势的未来展望。
