Kostic Lana, Sedov Egor, Soteriou Despina, Yosefzon Yahav, Fuchs Yaron
Laboratory of Stem Cell Biology and Regenerative Medicine, Department of Biology and Lokey Interdisciplinary Center for Life Sciences and Engineering, Technion Israel Institute of Technology, Haifa, Israel.
Curr Protoc Stem Cell Biol. 2017 May 16;41:1C.20.1-1C.20.11. doi: 10.1002/cpsc.26.
The epidermis consists of several distinct compartments including the interfollicular epidermis (IFE), sweat glands, sebaceous glands (SGs), and the hair follicle (HF). While the IFE and SGs are in a constant state of self-renewal, the HF cycles between phases of growth, destruction, and rest. The hair follicle stem cells (HFSCs) that fuel this perpetual cycle have been well described and are located in a niche termed the bulge. These bulge SCs express markers such as CD34 and Keratin 15 (K15), enabling the isolation of these cells. Here, we describe a powerful method for isolating HFSCs and epidermal progenitors from mouse skin utilizing fluorescence activated cell-sorting (FACS). Upon isolation, cells can be expanded and utilized in various in vivo and in vitro models aimed at studying the function of these unique cells. © 2017 by John Wiley & Sons, Inc.
表皮由几个不同的部分组成，包括毛囊间表皮（IFE）、汗腺、皮脂腺（SGs）和毛囊（HF）。虽然IFE和SGs处于持续自我更新的状态，但HF在生长、破坏和静止阶段之间循环。驱动这个持续循环的毛囊干细胞（HFSCs）已得到充分描述，它们位于一个称为隆突的生态位中。这些隆突干细胞表达诸如CD34和角蛋白15（K15）等标志物，使得能够分离这些细胞。在这里，我们描述了一种利用荧光激活细胞分选（FACS）从小鼠皮肤中分离HFSCs和表皮祖细胞的强大方法。分离后，细胞可以扩增并用于各种旨在研究这些独特细胞功能的体内和体外模型。© 2017约翰威立国际出版公司
