Sol Stefano, Antonini Dario, Missero Caterina
Department of Biology, University of Naples Federico II, Napoli, Italy.
CEINGE Biotecnologie Avanzate (Center for Genetic Engineering), Napoli, Italy.
Methods Mol Biol. 2019;1879:119-132. doi: 10.1007/7651_2018_131.
The interfollicular epidermis regenerates from a heterogeneous population of basal cells undergoing either self-renewal or terminal differentiation, thereby balancing cell loss in tissue turnover or in wound repair. In this chapter, we describe a reliable and simple method for isolating interfollicular epithelial stem cells from the skin of newborn mice or from tail and ear skin of adult mice using fluorescence-activated cell sorting (FACS). We also provide a detailed protocol for culturing interfollicular epidermal stem cells and to assess their proliferative potential and self-renewing ability. These techniques are useful for directly evaluating epidermal stem cell function in normal mice under different conditions or in genetically modified mouse models.
毛囊间表皮由经历自我更新或终末分化的异质性基底细胞群体再生，从而在组织更新或伤口修复中平衡细胞损失。在本章中，我们描述了一种可靠且简单的方法，即使用荧光激活细胞分选（FACS）从新生小鼠皮肤或成年小鼠尾巴和耳朵皮肤中分离毛囊间上皮干细胞。我们还提供了一份详细的方案，用于培养毛囊间表皮干细胞并评估其增殖潜力和自我更新能力。这些技术对于直接评估不同条件下正常小鼠或基因改造小鼠模型中表皮干细胞的功能很有用。
