Yada Ravi Chandra, Hong So Gun, Lin Yongshun, Winkler Thomas, Dunbar Cynthia E
Hematology Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health, Bethesda, Maryland.
iPSC Core, Center for Molecular Medicine, NHLBI, National Institutes of Health, Bethesda, Maryland.
Curr Protoc Stem Cell Biol. 2017 May 16;41:4A.11.1-4A.11.13. doi: 10.1002/cpsc.25.
The rhesus macaque (Macaca mulatta) is physiologically and phylogenetically similar to humans, and therefore represents an invaluable model for the pre-clinical assessment of the safety and feasibility of iPSC-derived cell therapies. The use of an excisable polycistronic lentiviral STEMCCA vector to reprogram rhesus fibroblasts or bone marrow stromal cells (BMSCs) into RhiPSCs is described. After reprogramming, the pluripotency transgenes can be removed by transient expression of Cre, leaving a residual genetic tag that may be useful for identification of RhiPSC-derived tissues in vivo. Finally, the steps to maintain pluripotency during passaging of RhiPSCs, required for successful utilization of RhiPSCs, is described. © 2017 by John Wiley & Sons, Inc.
恒河猴（猕猴）在生理和系统发育上与人类相似，因此是对诱导多能干细胞衍生的细胞疗法的安全性和可行性进行临床前评估的宝贵模型。本文描述了使用可切除的多顺反子慢病毒STEMCCA载体将恒河猴成纤维细胞或骨髓基质细胞（BMSC）重编程为恒河猴诱导多能干细胞（RhiPSC）的方法。重编程后，通过Cre的瞬时表达可以去除多能性转基因，留下一个残留的遗传标签，这可能有助于在体内识别RhiPSC衍生的组织。最后，本文描述了在RhiPSC传代过程中维持多能性的步骤，这是成功利用RhiPSC所必需的。© 2017 John Wiley & Sons, Inc.
