泰国伯克霍尔德菌：基因操作
Burkholderia thailandensis: Genetic Manipulation.
作者信息
Garcia Erin C
机构信息
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky.
出版信息
Curr Protoc Microbiol. 2017 May 16;45:4C.2.1-4C.2.15. doi: 10.1002/cpmc.27.
Abstract
Burkholderia thailandensis is a Gram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis. © 2017 by John Wiley & Sons, Inc.
摘要
泰国伯克霍尔德菌是一种革兰氏阴性细菌，原产于东南亚和澳大利亚北部的土壤。它是非致病性的；因此，它通常被用作相关人类病原体——鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌的模式生物。泰国伯克霍尔德菌相对容易进行基因操作，可以在这种生物体中使用多种强大的遗传工具。本单元描述了泰国伯克霍尔德菌中接合、自然转化、mini-Tn7插入和等位基因交换的方案。© 2017约翰威立父子公司。
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本文引用的文献
1
Tn7.
Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.MDNA3-0010-2014.
2
Sequence-defined transposon mutant library of Burkholderia thailandensis.
mBio. 2013 Nov 5;4(6):e00604-13. doi: 10.1128/mBio.00604-13.
3
Burkholderia BcpA mediates biofilm formation independently of interbacterial contact-dependent growth inhibition.
Mol Microbiol. 2013 Sep;89(6):1213-25. doi: 10.1111/mmi.12339. Epub 2013 Aug 16.
4
The Burkholderia bcpAIOB genes define unique classes of two-partner secretion and contact dependent growth inhibition systems.
PLoS Genet. 2012;8(8):e1002877. doi: 10.1371/journal.pgen.1002877. Epub 2012 Aug 9.
5
Dissection of the Burkholderia intracellular life cycle using a photothermal nanoblade.
Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):12095-100. doi: 10.1073/pnas.1107183108. Epub 2011 Jul 5.
6
Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068.
7
Stable, site-specific fluorescent tagging constructs optimized for burkholderia species.
Appl Environ Microbiol. 2010 Nov;76(22):7635-40. doi: 10.1128/AEM.01188-10. Epub 2010 Sep 17.
8
Versatile dual-technology system for markerless allele replacement in Burkholderia pseudomallei.
Appl Environ Microbiol. 2009 Oct;75(20):6496-503. doi: 10.1128/AEM.01669-09. Epub 2009 Aug 21.
9
Mutational analysis of Burkholderia thailandensis quorum sensing and self-aggregation.
J Bacteriol. 2009 Oct;191(19):5901-9. doi: 10.1128/JB.00591-09. Epub 2009 Jul 31.
10
Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates.
J Microbiol Methods. 2009 Mar;76(3):320-3. doi: 10.1016/j.mimet.2008.12.007. Epub 2008 Dec 29.
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