泰国伯克霍尔德菌：基因操作
Burkholderia thailandensis: Genetic Manipulation.
作者信息
Garcia Erin C
机构信息
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky.
出版信息
Curr Protoc Microbiol. 2017 May 16;45:4C.2.1-4C.2.15. doi: 10.1002/cpmc.27.
Abstract
Burkholderia thailandensis is a Gram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis. © 2017 by John Wiley & Sons, Inc.
摘要
泰国伯克霍尔德菌是一种革兰氏阴性细菌，原产于东南亚和澳大利亚北部的土壤。它是非致病性的；因此，它通常被用作相关人类病原体——鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌的模式生物。泰国伯克霍尔德菌相对容易进行基因操作，可以在这种生物体中使用多种强大的遗传工具。本单元描述了泰国伯克霍尔德菌中接合、自然转化、mini-Tn7插入和等位基因交换的方案。© 2017约翰威立父子公司。
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