Chen Hsuan, Chung Mei-Chu, Tsai Yuan-Ching, Wei Fu-Jin, Hsieh Jaw-Shu, Hsing Yue-Ie C
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115, Taiwan.
Department of Agronomy, National Taiwan University, Taipei, 106, Taiwan.
Bot Stud. 2015 Dec;56(1):22. doi: 10.1186/s40529-015-0103-9. Epub 2015 Sep 16.
The repeat sequences occupied more than 50 % of soybean genome. In order to understand where these repeat sequences distributed in soybean genome and its related Glycine species, we examined three new repeat sequences-soybean repeat sequence (SBRS1, SBRS2 and SBRS3), some nonspecific repeat sequences and 45S rDNA on several Glycine species, including annual and perennial accessions in this study. In the annual species, G. soja, signals for SBRS1 and ATT repeat can be found on each chromosome in GG genome, but those for SBRS2 and SBRS3 were located at three specific loci. In perennial Glycine species, these three SBR repeat frequently co-localized with 45S rDNA, two major 45S rDNA loci were found in all tetraploid species. However, an extra minor locus was found in one accession of the G. pescadrensis (Tab074), but not in another accession (Tab004). We demonstrate that some repetitive sequences are present in all Glycine species used in the study, but the abundancy is different in annual or perennial species. We suggest this study may provide additional information in investigations of the phylogeny in the Glycine species.
重复序列占大豆基因组的50%以上。为了了解这些重复序列在大豆基因组及其相关大豆属物种中的分布情况，我们在几种大豆属物种上检测了三种新的重复序列——大豆重复序列（SBRS1、SBRS2和SBRS3）、一些非特异性重复序列以及45S rDNA，其中包括本研究中的一年生和多年生材料。在一年生物种野生大豆中，GG基因组的每条染色体上都能找到SBRS1和ATT重复序列的信号，但SBRS2和SBRS3的信号位于三个特定位点。在多年生大豆属物种中，这三种SBR重复序列经常与45S rDNA共定位，在所有四倍体物种中都发现了两个主要的45S rDNA位点。然而，在一个野生大豆（Tab074）材料中发现了一个额外的次要位点，而在另一个材料（Tab004）中未发现。我们证明，本研究中使用的所有大豆属物种都存在一些重复序列，但一年生或多年生物种中的丰度不同。我们认为这项研究可能为大豆属物种系统发育研究提供更多信息。
