Yuan Jin-Ling, Sun Hui-Min, Guo Guang-Ping, Yue Jin-Jun, Gu Xiao-Ping
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang, China.
Bot Stud. 2014 Dec;55(1):4. doi: 10.1186/1999-3110-55-4. Epub 2014 Jan 15.
Chronological age is the primary consideration when studying the physiological development, aging, and flowering of bamboo. However, it's difficult to determine bamboo's chronological age if the time of germination is unknown. To investigate the chronological age of bamboo from the genomic DNA methylation profile, methylation-sensitive amplification polymorphism (MSAP) was employed to analyze the genomic DNA methylation of Moso bamboo (Phyllostachys heterocycla var. pubescens) from stands of nine germination-ages, using six primer pairs which have previously been shown to yield methylation rates that reflect the age of Moso bamboo.
The results showed that the total genomic DNA methylation rates in Moso bamboo at different chronological ages were significantly different, and the increase in genomic DNA methylation rate was consistent with the increase of chronological age. Six primer pairs displayed different genomic DNA methylation rates in Moso bamboo of nine age's group; however, a significantly positive correlation existed among these primer pairs. An integrated index was obtained by performing principal component analysis on the six primer pairs to represent the genomic DNA methylation levels in Moso bamboo of various chronological ages, and a quadratic curve between the chronological age and genomic DNA methylation levels was obtained.
Such a relationship between DNA methylation and its chronological age may serve a reference for its aging study in Moso bamboo.
在研究竹子的生理发育、衰老和开花时，时间年龄是主要考虑因素。然而，如果发芽时间未知，就很难确定竹子的时间年龄。为了从基因组DNA甲基化谱研究竹子的时间年龄，采用甲基化敏感扩增多态性（MSAP）方法，使用六对引物分析了九个发芽年龄林分的毛竹（Phyllostachys heterocycla var. pubescens）基因组DNA甲基化情况，这六对引物先前已被证明能产生反映毛竹年龄的甲基化率。
结果表明，不同时间年龄的毛竹基因组DNA总甲基化率存在显著差异，且基因组DNA甲基化率的增加与时间年龄的增加一致。六对引物在九个年龄组的毛竹中显示出不同的基因组DNA甲基化率；然而，这些引物对之间存在显著的正相关。通过对六对引物进行主成分分析得到一个综合指数，以代表不同时间年龄毛竹的基因组DNA甲基化水平，并得到了时间年龄与基因组DNA甲基化水平之间的二次曲线。
DNA甲基化与其时间年龄之间的这种关系可能为毛竹衰老研究提供参考。
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