Ni Jun, Shen Yan-Xia, Zhang Yan-Yan, Liu Yu
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou, China.
Bot Stud. 2014 Dec;55(1):42. doi: 10.1186/s40529-014-0042-x. Epub 2014 May 10.
Lateral root constitutes an important part of root system either in tap root plants or fibrous root plants. The development of lateral root primordium (LRP) in Arabidopsis, which has a tap root system with simple radial structure of primary root, has been well characterized. However, limited knowledge has been acquired on the plants with fibrous root system, such as rice. This is mainly due to their multiple cell layers coated on root, which disturb the observation of LRP.
We used an easy and quick method to strip the epidermal and cortex tissues of primary root so that the LRP can be easily observed under microscope. In this way, we observed the developmental processes of LRP in rice. In addition, we described the expression dynamics of several root development related genes, especially OsPINs (PIN-FORWMED), during the process of LRP development.
We reported an easy and quick method for LRP observation in rice and suggested a "fountain" model of auxin transport in LRP of rice, which is similar with that in Arabidopsis.
在直根系植物或须根系植物中，侧根都是根系的重要组成部分。拟南芥具有直根系，其主根的径向结构简单，侧根原基（LRP）的发育已得到充分表征。然而，对于像水稻这样具有须根系的植物，人们了解的知识有限。这主要是因为它们的根上覆盖有多层细胞，干扰了对侧根原基的观察。
我们采用了一种简便快捷的方法去除主根的表皮和皮层组织，以便在显微镜下轻松观察到侧根原基。通过这种方法，我们观察了水稻中侧根原基的发育过程。此外，我们描述了几个与根发育相关基因的表达动态，特别是在侧根原基发育过程中的OsPINs（PIN形成蛋白）。
我们报道了一种在水稻中观察侧根原基的简便快捷方法，并提出了水稻侧根原基中生长素运输的“喷泉”模型，该模型与拟南芥中的类似。
