Blilou Ikram, Xu Jian, Wildwater Marjolein, Willemsen Viola, Paponov Ivan, Friml Jirí, Heidstra Renze, Aida Mitsuhiro, Palme Klaus, Scheres Ben
Department of Molecular Genetics, Utrecht University, Padualaan 8, 3584CH Utrecht, The Netherlands.
Nature. 2005 Jan 6;433(7021):39-44. doi: 10.1038/nature03184.
Local accumulation of the plant growth regulator auxin mediates pattern formation in Arabidopsis roots and influences outgrowth and development of lateral root- and shoot-derived primordia. However, it has remained unclear how auxin can simultaneously regulate patterning and organ outgrowth and how its distribution is stabilized in a primordium-specific manner. Here we show that five PIN genes collectively control auxin distribution to regulate cell division and cell expansion in the primary root. Furthermore, the joint action of these genes has an important role in pattern formation by focusing the auxin maximum and restricting the expression domain of PLETHORA (PLT) genes, major determinants for root stem cell specification. In turn, PLT genes are required for PIN gene transcription to stabilize the auxin maximum at the distal root tip. Our data reveal an interaction network of auxin transport facilitators and root fate determinants that control patterning and growth of the root primordium.
植物生长调节剂生长素在拟南芥根中的局部积累介导了根的模式形成，并影响侧根和茎衍生原基的生长和发育。然而，生长素如何同时调节模式形成和器官生长，以及其分布如何以原基特异性方式稳定下来，仍不清楚。在这里，我们表明五个PIN基因共同控制生长素分布，以调节主根中的细胞分裂和细胞扩张。此外，这些基因的联合作用通过聚焦生长素最大值和限制多能性（PLT）基因的表达域，在模式形成中发挥重要作用，PLT基因是根干细胞特化的主要决定因素。反过来，PLT基因是PIN基因转录所必需的，以稳定根尖远端的生长素最大值。我们的数据揭示了一个生长素运输促进因子和根命运决定因素的相互作用网络，该网络控制根原基的模式形成和生长。
