• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

镰刀菌酸诱导番茄细胞死亡及氧化代谢变化

Fusaric acid induced cell death and changes in oxidative metabolism of Solanum lycopersicum L.

作者信息

Singh Vivek Kumar, Upadhyay Ram Sanmukh

机构信息

Laboratory of Mycopathology and Microbial Technology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, India.

出版信息

Bot Stud. 2014 Dec;55(1):66. doi: 10.1186/s40529-014-0066-2. Epub 2014 Aug 27.

DOI:10.1186/s40529-014-0066-2
PMID:28510945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5432760/
Abstract

BACKGROUND

Fusaric acid (FA) has been shown to stimulate the rapid development of disease symptoms, such as necrosis and foliar desiccation. In this study, we have evaluated the phytotoxicity of FA on tomato plants (Solanum lycopersicum L.). FA induced necrotic lesions in detached leaves, which are reminiscent of hypersensitive response (HR) lesions induced by plant-pathogen interactions and other abiotic stress factors.

RESULTS

FA-treated tomato leaves exhibited visible necrotic lesion as a result of cell death which was evident by Evans blue staining, enhanced reactive oxygen species (ROS) levels and DNA degradation. Changes in the generation of O and HO as well as the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were examined in FA-treated tomato leaves. It was observed that FA exposure stimulated oxidative burst in the leaves, resulting in a lasting activation of O and HO production. After first day of FA application, the HO scavenging enzymes CAT and APX showed a strong activity decrease followed by gradual recovery to the control level after 2 and 3 days.

CONCLUSION

A concomitant increase in ROS production, the down regulation of antioxidative enzymes activities and upregulation of lipid peroxidation were crucial for the onset of cell death. These results suggested that FA-induced damage might result from ROS pathways. Thus, our experiments provide a useful model plant system for research on FA-induced plant cell death.

摘要

背景

已表明镰孢菌酸（FA）能刺激疾病症状快速发展，如坏死和叶片干枯。在本研究中，我们评估了FA对番茄植株（番茄）的植物毒性。FA在离体叶片中诱导坏死斑，这让人联想到植物 - 病原体相互作用及其他非生物胁迫因素诱导的过敏反应（HR）损伤。

结果

经FA处理的番茄叶片因细胞死亡出现可见的坏死斑，伊文思蓝染色、活性氧（ROS）水平升高和DNA降解均证实了这一点。检测了经FA处理的番茄叶片中O和HO的生成变化以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）的活性。观察到FA处理刺激叶片中的氧化爆发，导致O和HO生成持续激活。在施用FA的第一天后，HO清除酶CAT和APX活性大幅下降，随后在2天和3天后逐渐恢复到对照水平。

结论

ROS生成增加、抗氧化酶活性下调以及脂质过氧化上调共同作用对细胞死亡的发生至关重要。这些结果表明FA诱导的损伤可能源于ROS途径。因此，我们的实验为研究FA诱导的植物细胞死亡提供了一个有用的模式植物系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/d8d4669ce510/40529_2014_Article_9066_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/d63a6b52e453/40529_2014_Article_9066_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/ca251f015560/40529_2014_Article_9066_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/baa9a4c7050a/40529_2014_Article_9066_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/d47ffd7d76c0/40529_2014_Article_9066_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/edda7fd93514/40529_2014_Article_9066_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/cff4336a0eb1/40529_2014_Article_9066_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/b7b16d69f693/40529_2014_Article_9066_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/d8d4669ce510/40529_2014_Article_9066_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/d63a6b52e453/40529_2014_Article_9066_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/ca251f015560/40529_2014_Article_9066_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/baa9a4c7050a/40529_2014_Article_9066_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/d47ffd7d76c0/40529_2014_Article_9066_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/edda7fd93514/40529_2014_Article_9066_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/cff4336a0eb1/40529_2014_Article_9066_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/b7b16d69f693/40529_2014_Article_9066_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/5432760/d8d4669ce510/40529_2014_Article_9066_Fig8_HTML.jpg

相似文献

1
Fusaric acid induced cell death and changes in oxidative metabolism of Solanum lycopersicum L.镰刀菌酸诱导番茄细胞死亡及氧化代谢变化
Bot Stud. 2014 Dec;55(1):66. doi: 10.1186/s40529-014-0066-2. Epub 2014 Aug 27.
2
Ethylene-dependent regulation of oxidative stress in the leaves of fusaric acid-treated tomato plants.镰刀菌酸处理的番茄植株叶片中乙烯依赖的氧化应激调节
Plant Physiol Biochem. 2023 Mar;196:841-849. doi: 10.1016/j.plaphy.2023.02.047. Epub 2023 Feb 27.
3
Role of fusaric acid in the development of 'Fusarium wilt' symptoms in tomato: Physiological, biochemical and proteomic perspectives.腐马酸在番茄“枯萎病”症状发展中的作用：生理学、生物化学和蛋白质组学视角
Plant Physiol Biochem. 2017 Sep;118:320-332. doi: 10.1016/j.plaphy.2017.06.028. Epub 2017 Jun 28.
4
Prolonged dark period modulates the oxidative burst and enzymatic antioxidant systems in the leaves of salicylic acid-treated tomato.延长的黑暗期调节水杨酸处理的番茄叶片中的氧化爆发和酶促抗氧化系统。
J Plant Physiol. 2017 Jun;213:216-226. doi: 10.1016/j.jplph.2017.03.013. Epub 2017 Mar 23.
5
Comparative Evaluation of Biochemical Changes in Tomato ( Mill.) Infected by and Its Toxic Metabolites (TeA, AOH, and AME).番茄（Mill.）受[病原菌名称未给出]及其毒性代谢产物（TeA、AOH和AME）感染后的生化变化比较评估
Front Plant Sci. 2016 Sep 22;7:1408. doi: 10.3389/fpls.2016.01408. eCollection 2016.
6
Pesticide-induced oxidative stress and antioxidant responses in tomato (Solanum lycopersicum) seedlings.农药诱导番茄（Solanum lycopersicum）幼苗的氧化应激和抗氧化反应。
Ecotoxicology. 2018 Sep;27(7):919-935. doi: 10.1007/s10646-018-1916-6. Epub 2018 Mar 1.
7
Ascorbate glutathione antioxidant system alleviates fly ash stress by modulating growth physiology and biochemical responses in .抗坏血酸-谷胱甘肽抗氧化系统通过调节生长生理和生化反应来缓解粉煤灰胁迫。
Saudi J Biol Sci. 2022 Mar;29(3):1322-1336. doi: 10.1016/j.sjbs.2021.12.013. Epub 2021 Dec 11.
8
Mycotoxin Ochratoxin A-induced cell death and changes in oxidative metabolism of Arabidopsis thaliana.黄曲霉毒素 Ochratoxin A 诱导的拟南芥细胞死亡和氧化代谢变化。
Plant Cell Rep. 2010 Feb;29(2):153-61. doi: 10.1007/s00299-009-0808-x. Epub 2009 Dec 29.
9
Fusaric acid-evoked oxidative stress affects plant defence system by inducing biochemical changes at subcellular level.富马酸诱导的氧化应激通过在亚细胞水平诱导生化变化来影响植物防御系统。
Plant Cell Rep. 2023 Dec 18;43(1):2. doi: 10.1007/s00299-023-03084-9.
10
Polyamine Oxidase Triggers HO-Mediated Spermidine Improved Oxidative Stress Tolerance of Tomato Seedlings Subjected to Saline-Alkaline Stress.多胺氧化酶触发 HO 介导的亚精胺提高番茄幼苗耐盐碱性氧化应激。
Int J Mol Sci. 2022 Jan 30;23(3):1625. doi: 10.3390/ijms23031625.

引用本文的文献

1
Bacillus endophytic strains control Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici in tomato cv. Perinha.内生芽孢杆菌菌株可防治番茄品种佩里尼亚中由尖孢镰刀菌番茄专化型引起的枯萎病。
Braz J Microbiol. 2024 Dec;55(4):4019-4034. doi: 10.1007/s42770-024-01539-z. Epub 2024 Oct 22.
2
Identification of hub genes and potential networks by centrality network analysis of PCR amplified Fusarium oxysporum f. sp. lycopersici EF1α gene.利用 Fusarium oxysporum f. sp. lycopersici EF1α 基因的中心网络分析鉴定枢纽基因和潜在网络。
BMC Microbiol. 2024 Sep 11;24(1):336. doi: 10.1186/s12866-024-03434-x.
3

本文引用的文献

1
The role of active oxygen in the response of plants to water deficit and desiccation.活性氧在植物对水分亏缺和干燥的响应中的作用。
New Phytol. 1993 Sep;125(1):27-58. doi: 10.1111/j.1469-8137.1993.tb03863.x.
2
Ozone-induced oxidative burst in the ozone biomonitor plant, tobacco Bel W3.臭氧生物监测植物烟草Bel W3中臭氧诱导的氧化爆发
Plant J. 1998 Oct;16(2):235-45. doi: 10.1046/j.1365-313x.1998.00294.x.
3
Classes of programmed cell death in plants, compared to those in animals.植物细胞程序性死亡的类型，与动物中的相比。
Exogenous application of mycotoxin fusaric acid improve the morphological, cytogenetic, biochemical and anatomical parameters in salt (NaCl) stressed Allium cepa L.
外源施加真菌毒素镰刀菌酸可改善盐（NaCl）胁迫下洋葱的形态、细胞遗传学、生物化学和解剖学参数。
Sci Rep. 2024 Mar 23;14(1):6962. doi: 10.1038/s41598-024-57762-z.
4
Genetic dissection of the degradation pathways for the mycotoxin fusaric acid in T16.真菌毒素伏马菌素降解途径的遗传剖析在 T16 中。
Appl Environ Microbiol. 2023 Dec 21;89(12):e0063023. doi: 10.1128/aem.00630-23. Epub 2023 Dec 6.
5
Engineered coumarin accumulation reduces mycotoxin-induced oxidative stress and disease susceptibility.工程化香豆素积累可降低真菌毒素诱导的氧化应激和疾病易感性。
Plant Biotechnol J. 2023 Dec;21(12):2490-2506. doi: 10.1111/pbi.14144. Epub 2023 Aug 14.
6
Metabolite Formation by Fungal Pathogens of Potatoes ( L.) in the Presence of Bioprotective Agents.在生物保护剂存在的情况下，真菌病原体对马铃薯（L.）的代谢产物形成。
Int J Environ Res Public Health. 2023 Mar 22;20(6):5221. doi: 10.3390/ijerph20065221.
7
Alkaline pH, Low Iron Availability, Poor Nitrogen Sources and CWI MAPK Signaling Are Associated with Increased Fusaric Acid Production in .碱性 pH 值、低铁可用性、不良氮源和 CWI MAPK 信号与. 中增加的伏马菌素产量有关。
Toxins (Basel). 2023 Jan 6;15(1):50. doi: 10.3390/toxins15010050.
8
Defence response in plants and animals against a common fungal pathogen.植物和动物对常见真菌病原体的防御反应。
Curr Res Microb Sci. 2022 Apr 19;3:100135. doi: 10.1016/j.crmicr.2022.100135. eCollection 2022.
9
Physiological Response of Cape Gooseberry Plants to f. sp. , Fusaric Acid, and Water Deficit in a Hydrophonic System.酸浆属植物在水培系统中对茄病镰刀菌、镰刀菌酸和水分亏缺的生理响应。
Front Plant Sci. 2021 Aug 5;12:702842. doi: 10.3389/fpls.2021.702842. eCollection 2021.
10
Characterization of the Exo-Metabolome of the Emergent Phytopathogen sp. nov., a Causal Agent of Dieback.新兴植物病原菌 sp. nov. 的胞外代谢组学特征，该病原菌是萎蔫病的致病因子。
Toxins (Basel). 2021 Apr 9;13(4):268. doi: 10.3390/toxins13040268.
J Exp Bot. 2011 Oct;62(14):4749-61. doi: 10.1093/jxb/err196. Epub 2011 Jul 21.
4
Ochratoxin A induces apoptosis in neuronal cells.赭曲霉毒素 A 诱导神经元细胞凋亡。
Genes Nutr. 2009 Mar;4(1):41-8. doi: 10.1007/s12263-008-0109-y. Epub 2009 Jan 16.
5
Active oxygen in plant pathogenesis.植物发病机制中的活性氧
Annu Rev Phytopathol. 1995;33:299-321. doi: 10.1146/annurev.py.33.090195.001503.
6
Reactive oxygen species as signals that modulate plant stress responses and programmed cell death.活性氧作为调节植物应激反应和程序性细胞死亡的信号。
Bioessays. 2006 Nov;28(11):1091-101. doi: 10.1002/bies.20493.
7
Reactive oxygen species in plant cell death.植物细胞死亡中的活性氧
Plant Physiol. 2006 Jun;141(2):384-90. doi: 10.1104/pp.106.078295.
8
Effects of fusaric Acid on tomato root hair membrane potentials and ATP levels.腐马酸对番茄根毛膜电位和ATP水平的影响。
Plant Physiol. 1984 Jan;74(1):39-42. doi: 10.1104/pp.74.1.39.
9
The Occurrence of Peroxide in a Perennial Plant, Populus gelrica.柳树中过氧化物的出现
Plant Physiol. 1976 Feb;57(2):308-9. doi: 10.1104/pp.57.2.308.
10
Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5.重金属胁迫对钝顶螺旋藻-S5中脯氨酸、丙二醛和超氧化物歧化酶活性的影响。
Ecotoxicol Environ Saf. 2007 Feb;66(2):204-9. doi: 10.1016/j.ecoenv.2006.02.002. Epub 2006 Apr 5.