Tomato plants ( L.) were developed in soils with different fly ash (FA) amendments (25, 50, 75, 100% FA) to measure the effects of FA on metal accumulation, chlorophyll pigments, chlorophyll fluorescence, growth, biomass, gas exchange parameters, and the ascorbate glutathione pathway (AsA-GSH). The metal concentration was much higher in FA compared to the garden soil/(control). The observed metal translocation was higher in roots than shoots. Plants raised in soils treated with 50% or more FA showed significant decreases in growth, biomass, gas exchange parameters, protein, chlorophyll pigments, and fluorescence parameters. Additionally, a significant increase in antioxidants under higher FA-amended soils were observed. Our results showed that the ability of plants to effectively synchronize the actions of antioxidant enzymes associated in reactive oxygen species (ROS) scavenging - notably superoxidase dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR) - with good maintenance of the AsA/DHA ratio, that could be connected to FA stress tolerance. The toxic metals present in FA caused oxidative stress in , as evident from the increase in electrolyte leakage (EL), lipid peroxidation (MDA), and ROS levels. Furthermore, the AsA-GSH cycle plays a key role in alleviating oxidative damage caused by FA application.