Moriki T, Kimura S, Yamane T, Hara H
Department of Pathology, Kochi Medical School, Japan.
Acta Pathol Jpn. 1988 Jun;38(6):693-704. doi: 10.1111/j.1440-1827.1988.tb02341.x.
The purpose of this study was to examine the distribution pattern of acridine orange (AO) binding to DNA in transformed cells and derived tumor cells. Two cell clones from hamster embryonic fibroblasts (HEF) transformed by herpes simplex virus type 2, tumor cells derived from the transformed cell clones, and normal HEF in culture were processed for ultracytochemistry. Uptake studies and autoradiography using [3H] thymidine and [3H]-uridine were also performed. Ultrastructural examination revealed that AO became bound to DNA exclusively within the euchromatin portion of the cell nucleus. The percentages of AO-positive cell nuclei were 18.0% and 15.5% in transformed cell clones and 21.3% and 18.3% in the derived tumor cells, but only 2. 7% in the HEF. The average amounts of AO-chromatin interaction products increased in the transformed and tumor cells. Labeling indices of [3H] thymidine were 28.9% and 33.2% in both the transformed cell clones, 32.4% and 36.9% in their tumor cells, and 3.7% in the HEF. Euchromatin/heterochromatin (EU/HEF) ratios were found to be higher in AO-positive nuclei than in AO-negative nuclei in each cell line. The increase in the number of AO-positive nuclei and in the EU/HET ratios may represent a basic reaction of cell nuclei in transformation and tumorigenesis.
本研究的目的是检测吖啶橙（AO）与转化细胞及衍生肿瘤细胞中DNA的结合分布模式。对来自经2型单纯疱疹病毒转化的仓鼠胚胎成纤维细胞（HEF）的两个细胞克隆、源自转化细胞克隆的肿瘤细胞以及培养中的正常HEF进行超微细胞化学处理。还进行了使用[3H]胸腺嘧啶核苷和[3H]尿苷的摄取研究及放射自显影。超微结构检查显示，AO仅在细胞核的常染色质部分与DNA结合。转化细胞克隆中AO阳性细胞核的百分比分别为18.0%和15.5%，衍生肿瘤细胞中为21.3%和18.3%，而HEF中仅为2.7%。转化细胞和肿瘤细胞中AO-染色质相互作用产物的平均量增加。[3H]胸腺嘧啶核苷的标记指数在转化细胞克隆中分别为28.9%和33.2%，在其肿瘤细胞中为32.4%和36.9%，在HEF中为3.7%。发现每个细胞系中AO阳性细胞核的常染色质/异染色质（EU/HEF）比率高于AO阴性细胞核。AO阳性细胞核数量及EU/HET比率的增加可能代表细胞核在转化和肿瘤发生过程中的一种基本反应。
