Hara H, Moriki T, Matsusaki K, Hiroi M, Yamane T
Acta Pathol Jpn. 1984 Sep;34(5):1041-7. doi: 10.1111/j.1440-1827.1984.tb07634.x.
The ultracytochemical acridine orange (AO) method has been employed to demonstrate DNA template activity within untreated human brain tumors. A total of 16 biopsies from brain tumors removed for frozen section examination were investigated. Ultrastructural examination revealed that AO binds to DNA exclusively within the extended euchromatin portion of the cell nucleus of brain tumors. The percentage of AO positive cells varied from 6.0 to 48.6% in various types of brain tumors. A few AO reaction products were visible in the nuclei of the endothelial cells and in the pericytes of the capillaries within neoplastic tissue. The present results suggested that untreated human brain tumors exhibit de-repression of DNA template normally repressed in the adult state. This altered DNA templates may be related to abnormal cell proliferation and differentiation diverted from the normal state.
采用超细胞化学吖啶橙（AO）法来证明未经处理的人脑肿瘤内的DNA模板活性。对因冷冻切片检查而切除的脑肿瘤的16份活检标本进行了研究。超微结构检查显示，AO仅与脑肿瘤细胞核中伸展的常染色质部分内的DNA结合。在各种类型的脑肿瘤中，AO阳性细胞的百分比在6.0%至48.6%之间变化。在肿瘤组织内的内皮细胞核和毛细血管的周细胞中可见少数AO反应产物。目前的结果表明，未经处理的人脑肿瘤表现出在成年状态下通常被抑制的DNA模板的去抑制。这种改变的DNA模板可能与偏离正常状态的异常细胞增殖和分化有关。
