Gotzos V, Cappelli-Gotzos B, Conti G, Petropoulos P
Institute of Histology and General Embryology, University of Frebourg, Switzerland.
Anticancer Res. 1988 Nov-Dec;8(6):1201-10.
The cells from a malignant fibrous histiocytoma were enzymatically isolated and cultured in vitro. The cultures were observed by microcinematography and with an electron microscope after fixation and embedding. The interactions between histiocytic and tumor cells resulted in tumor cell death. The microcinematographic study revealed that filiform projections of the histiocytic cell protrude toward the tumor cell surface making contacts for varying periods of time. The tumor cell then contracts and almost simultaneously some granules (lysosomes?) appear in the tumor cell cytoplasm. These granules eventually fuse with each other thus increasing in volume. The resulting granulation heads for the periphery of the tumor cell at a site previously touch by a projection of a histiocytic cell; at this site the membrane bursts and the tumor cell dies leaking amorphous cytoplasmic material. Before bursting the tumor cell does not show any noticeable morphological changes.
从恶性纤维组织细胞瘤中分离出细胞并进行体外酶解培养。培养物经固定、包埋后，用显微电影摄影术和电子显微镜进行观察。组织细胞与肿瘤细胞之间的相互作用导致肿瘤细胞死亡。显微电影摄影研究显示，组织细胞的丝状突起伸向肿瘤细胞表面并与之接触不同时间。然后肿瘤细胞收缩，几乎同时在肿瘤细胞胞质中出现一些颗粒（溶酶体？）。这些颗粒最终相互融合，体积增大。形成的颗粒团朝向肿瘤细胞周边的一个部位，该部位先前被组织细胞的一个突起接触过；在此部位，细胞膜破裂，肿瘤细胞死亡，泄漏出无定形的细胞质物质。在破裂之前，肿瘤细胞未显示出任何明显的形态变化。
