a Department of Physiology, School of Medicine , Shiraz University of Medical Sciences , Shiraz , Iran.
b Department of Food and Drug, Reference Laboratory, School of Pharmacy , Shiraz University of Medical Sciences , Shiraz , Iran.
Clin Exp Hypertens. 2017;39(4):344-349. doi: 10.1080/10641963.2016.1259329. Epub 2017 May 17.
Both renal and respiratory diseases are common with high mortality rate around the world. This study was the first to compare effects of two kidneys, one clip (2K1C) and one-kidney, one clip (1K1C) Goldblatt hypertension on right ventricular pressure during normal condition and mechanical ventilation with hypoxia gas. Male Sprague-Dawley rats were subjected to control, 2K1C, or 1K1C groups. Twenty-eight days after the first surgery, animals were anesthetized, and femoral artery and vein, and right ventricle cannulated. Systemic arterial pressure and right ventricular systolic pressures (RVSP) were recorded during ventilation the animals with normoxic or hypoxic gas. RVSP in the 1K1C group was significantly more than the control and 2K1C groups during baseline conditions and ventilation the animals with hypoxic gas. Administration of antioxidant Trolox increased RVSP in the 1K1C and control groups compared with their baselines. Furthermore, there was no alteration in RVSP during hypoxia in the presence of Trolox. This study indicated that RVSP only increased after 28 days induction of 1K1C but not 2K1C model. In addition, it seems that the response to hypoxic gas and antioxidants in 1K1C is more than 2K1C. These data also suggest that effects of 1K1C may partially be related to reactive oxygen species (ROS) pathways.
肾和呼吸系统疾病在全球范围内都很常见，死亡率很高。本研究首次比较了两种肾脏夹闭（2K1C）和单侧肾脏夹闭（1K1C）Goldblatt 高血压模型在正常条件和低氧气体机械通气下对右心室压力的影响。雄性 Sprague-Dawley 大鼠分为对照组、2K1C 组和 1K1C 组。第一次手术后 28 天，对动物进行麻醉，并插入股动脉和股静脉以及右心室导管。在给动物通气时记录系统动脉压和右心室收缩压（RVSP），通气气体为常氧或低氧。在基础状态和低氧气体通气下，1K1C 组的 RVSP 明显高于对照组和 2K1C 组。给予抗氧化剂 Trolox 后，1K1C 组和对照组的 RVSP 均高于其基础值。此外，在 Trolox 存在的情况下，低氧对 RVSP 没有改变。本研究表明，只有在 1K1C 诱导 28 天后 RVSP 才会升高，而 2K1C 模型则不会。此外，1K1C 对低氧气体和抗氧化剂的反应似乎比 2K1C 更强。这些数据还表明，1K1C 的作用可能部分与活性氧（ROS）途径有关。
