Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo. Av. Bandeirantes, 3900, CEP 14040-901, Ribeirão Preto, SP, Brazil.
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo. Av. Prof. Zeferino Vaz s/n, Monte Alegre, CEP 14040-903, Ribeirão Preto, SP, Brazil and Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo. Av. Bandeirantes, 3900, CEP 14040-901, Ribeirão Preto, SP, Brazil.
Photochem Photobiol Sci. 2017 Jul 1;16(7):1071-1078. doi: 10.1039/c6pp00306k. Epub 2017 May 17.
The cytotoxicity of nitrofurantoin (NFT) in the dark and after light exposure (UVA irradiation, λ = 385 nm) was evaluated in murine melanoma B16F10 cells. NFT induces both cell proliferation and inhibition of cell viability. The dominance of one or the other effect depends on the drug concentration, incubation time (t) and irradiation dose. The uptake of NFT in these cells, as well as its photocytotoxicity, reaches saturation after 24 hours of incubation. The mechanism of cell death in the dark is associated with the enzymatic release of nitric oxide (NO). The increase of NFT cytotoxicity under light irradiation is associated with the increase of NO concentration due to photorelease. NO photorelease by NFT in solution was confirmed by chemiluminescence, while NO formation in cells was confirmed by fluorescence microscopy using DAF-2DA, a specific indicator of NO in living cells. The NFT does not enter nuclei, distributing preferentially in the cell cytoplasm, as shown by fluorescence microscopy.
在黑暗中和光暴露（UVA 照射，λ = 385nm）后，评估了呋喃妥因（NFT）对鼠黑色素瘤 B16F10 细胞的细胞毒性。NFT 诱导细胞增殖和抑制细胞活力。一种或另一种效应的优势取决于药物浓度、孵育时间（t）和辐照剂量。这些细胞中 NFT 的摄取及其光细胞毒性在孵育 24 小时后达到饱和。黑暗中细胞死亡的机制与一氧化氮（NO）的酶释放有关。在光照下，由于光释放，NFT 的细胞毒性增加。通过化学发光证实了 NFT 在溶液中的 NO 光释放，而通过荧光显微镜使用 DAF-2DA（活细胞中 NO 的特异性指示剂）证实了细胞中 NO 的形成。NFT 不进入细胞核，如荧光显微镜所示，优先分布在细胞质中。
