在水凝胶中用电泳纳米颗粒解压缩和剪切 DNA。
Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels.
机构信息
Department of Robotics, Graduate School of Engineering, Tohoku University, 6-6-1 Aobayama, Sendai 980-8579, Japan.
出版信息
Phys Chem Chem Phys. 2017 May 31;19(21):13414-13418. doi: 10.1039/c7cp02214j.
We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.
我们展示了通过电控制将一段与水凝胶结合的 DNA 双链体进行解链和剪切去杂交。通过电泳（施加 25Vcm 的电场）拉动金纳米颗粒来施加张力，从而拉动 DNA 双链体。被拉动的 DNA 链逐渐从水凝胶中释放出来。解链的释放速度快于剪切；例如，对于 15 个碱基对的双链体，速度快 3 倍，这有助于设计电驱动的 DNA 器件。
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