Jones B, Carding S, Kyes S, Mjolsness S, Janeway C, Hayday A
Department of Biology, Yale University, New Haven, CT 06511.
Eur J Immunol. 1988 Dec;18(12):1907-15. doi: 10.1002/eji.1830181207.
Northern analysis, hybridization in situ and cDNA sequence analysis have been used to demonstrate that the induction of T cell gamma-gene expression is a general occurrence when primary splenic T cells of adult mice are cultured in short-term mixed lymphocyte reactions (MLR). Splenic T cells from nine strains of mice examined in eleven different MLR all showed significant induction of gamma-RNA, even when the primary T cell response was to only a three amino acid mismatch in a major histocompatibility complex class I antigen. In MLR examined in detail, the expression is highly enriched for in CD3+ "double-negative" T cells (lacking both CD4 and CD8 expression). A cDNA sequence analysis, constituting the first such analysis of any size of gamma-gene transcripts from circulating, peripheral cells of adult mice, revealed transcription to be frequently of productively rearranged genes. These genes display extensive junctional diversity.
Northern印迹分析、原位杂交和cDNA序列分析已被用于证明，当成年小鼠的原代脾T细胞在短期混合淋巴细胞反应（MLR）中培养时，T细胞γ基因表达的诱导是普遍存在的。在11种不同的MLR中检测的9个品系小鼠的脾T细胞均显示出γ-RNA的显著诱导，即使原代T细胞反应仅针对主要组织相容性复合体I类抗原中的三个氨基酸错配。在详细检测的MLR中，CD3⁺“双阴性”T细胞（缺乏CD4和CD8表达）中的表达高度富集。cDNA序列分析是对成年小鼠循环外周细胞中任何大小的γ基因转录本进行的首次此类分析，结果显示转录通常来自有效重排的基因。这些基因表现出广泛的连接多样性。
