Godavarthi S, Mohan Kumar K, Vázquez Vélez E, Hernandez-Eligio A, Mahendhiran M, Hernandez-Como N, Aleman M, Martinez Gomez L
Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, 62210 Cuernavaca, MOR, Mexico; Conacyt - Division Academica de Ciencias Basicas, Universidad Juarez Autonoma de Tabasco, Km 1 Carretera Cunduacan, Jalpa de Mendez A.P 24 C.P 86690, Colonia Esmeralda, Cunduacan, Tabasco, Mexico.
Universidad Nacional Autonoma de Mexico, Instituto de Ciencias Fisicas, Avenida Universidad s/n, 62210 Cuernavaca, MOR, Mexico.
J Photochem Photobiol B. 2017 Jul;172:36-41. doi: 10.1016/j.jphotobiol.2017.05.014. Epub 2017 May 10.
This work focused on the use of waste seaweed Sargassum fluitans (S. fluitans) as carbon source precursor to prepare nitrogen doped carbon dots (NCDs) by hydrothermal method. High resolution transmission electron microscopic (HR-TEM) studies revealed that the synthesized water soluble NCDs are in the size range of 2-8nm and exhibits excellent fluorescent properties with a quantum yield of 18.2%. Elemental nitrogen in NCDs was evidenced by X-ray photoelectron spectroscopy (XPS) and Fourier transformed infrared spectrum (FT-IR). The phytochemical analysis of S. fluitans using H NMR and C NMR revealed the presence of few amino acids which act as nitrogen source in the preparation of NCDs. Application of NCDs as fluorophore for double stranded DNA, single stranded DNA and RNA detection was highlighted in this study. Excellent fluorescent tagging abilities of NCDs with the biological nucleic acids were evidenced using gel electrophoresis. Significant increase in fluorescence was observed upon tagging of NCDs with nucleic acids and this particular phenomenon helps better in visualizing the nucleic acids. All three nucleic acids i.e. double stranded DNA, single stranded DNA and RNA showed similar phenomenon upon tagging with NCDs. Thus synthesized NCDs may be used as an alternate fluorophore for commercial toxic organic staining agents to visualize nucleic acids.
这项工作聚焦于利用废弃海藻漂浮马尾藻（S. fluitans）作为碳源前驱体，通过水热法制备氮掺杂碳点（NCDs）。高分辨率透射电子显微镜（HR-TEM）研究表明，合成的水溶性NCDs尺寸在2 - 8纳米范围内，具有优异的荧光特性，量子产率为18.2%。X射线光电子能谱（XPS）和傅里叶变换红外光谱（FT-IR）证实了NCDs中存在元素氮。利用氢核磁共振（H NMR）和碳核磁共振（C NMR）对漂浮马尾藻进行的植物化学分析表明，存在少量氨基酸，这些氨基酸在制备NCDs时充当氮源。本研究突出了NCDs作为荧光团用于双链DNA、单链DNA和RNA检测的应用。使用凝胶电泳证明了NCDs与生物核酸具有出色的荧光标记能力。在用核酸标记NCDs后观察到荧光显著增加，这种特殊现象有助于更好地可视化核酸。所有三种核酸，即双链DNA、单链DNA和RNA在用NCDs标记时都表现出类似现象。因此，合成的NCDs可作为商业有毒有机染色剂的替代荧光团来可视化核酸。
