Sharma Neha, Lee Hae-Jeung
Department of Food and Nutrition, College of Bionanotechnology, Gachon University, Seongnam-si 13120, Republic of Korea.
Institute for Aging and Clinical Nutrition Research, Gachon University, Seongnam-si 13120, Gyeonggi-do, Republic of Korea.
Biosensors (Basel). 2025 Feb 12;15(2):105. doi: 10.3390/bios15020105.
Carbon dots (CDs) derived from biomass are promising fluorescent probes for specific analyte detection due to their specificity, biocompatibility, selectivity, and sensitivity. In this work, carbon dots were prepared hydrothermally from natural material, fruits (hereafter referred to as MPCDs), without adding any chemicals. The prepared MPCDs were characterized using optical, microscopic, and spectroscopic methods that revealed the presence of numerous functional groups and fluorescent properties. MPCDs exhibited exceptional characteristics such as water solubility, photostability, excitation-dependent fluorescence emission, and ionic stability. Transmission electron microscopy found that the average size of the MPCDs was 8 nm. MPCDs exhibited remarkable sensing ability for hemin, with a good linearity (R = 0.999) and a lower limit of detection of 14.1 nM. MPCDs demonstrated fluorescence quenching-based detection of hemin, primarily owing to ground state complex formation and the inner filter effect. Furthermore, the prepared material exhibited excellent antioxidant potential against 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl radicals with EC values of 25.4 and 205.4 µg/mL, respectively. The study suggests that CDs from fruits could be used as optical sensors for hemin detection as well as to scavenge selected radicals.
源自生物质的碳点（CDs）由于其特异性、生物相容性、选择性和灵敏度，是用于特定分析物检测的有前途的荧光探针。在这项工作中，不添加任何化学物质，通过水热法从天然材料水果中制备了碳点（以下简称MPCDs）。使用光学、显微镜和光谱方法对制备的MPCDs进行了表征，结果显示存在大量官能团和荧光特性。MPCDs表现出优异的特性，如水溶性、光稳定性、激发依赖性荧光发射和离子稳定性。透射电子显微镜发现MPCDs的平均尺寸为8纳米。MPCDs对血红素表现出显著的传感能力，具有良好的线性关系（R = 0.999），检测下限为14.1 nM。MPCDs证明了基于荧光猝灭的血红素检测方法，主要是由于基态复合物的形成和内滤光效应。此外，制备的材料对2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）和2,2-二苯基-1-苦基肼自由基表现出优异的抗氧化潜力，其EC值分别为25.4和205.4 µg/mL。该研究表明，来自水果的碳点可作为用于血红素检测的光学传感器以及清除选定自由基的物质。
