Peele D B, Farmer J D, MacPhail R C
Northrop Services, Inc., Environmental Sciences, Research Triangle Park, North Carolina 27709.
Fundam Appl Toxicol. 1988 Oct;11(3):416-28. doi: 10.1016/0272-0590(88)90106-6.
The conditioned flavor-aversion paradigm was used to assess the toxicity of acutely administered cadmium and the interaction of cadmium with the heavy-metal chelating agents dimercaprol (BAL) and dimercaptosuccinic acid (DMSA). Shortly after consuming saccharin, rats received ip administration of cadmium either alone or in combination with sc administration of BAL or DMSA. Three days later they were given the choice between consuming saccharin or water, and saccharin preferences were recorded. When compared to rats receiving either nothing or the vehicle, rats receiving cadmium displayed significant reductions in saccharin preference (i.e., conditioned flavor aversions). BAL and DMSA were also capable of producing conditioned flavor aversions when given alone. Rats receiving cadmium in combination with either BAL or DMSA displayed significant, but not complete attenuations of conditioned flavor aversions when compared to the flavor aversions of rats receiving cadmium alone. Chelator-induced blockade of cadmium-induced flavor-aversion conditioning was not obtained when BAL or DMSA administration was delayed by 4 hr. Attenuation of cadmium-induced aversions by BAL and DMSA extends earlier findings of an attenuation of lead-induced flavor-aversion conditioning by these complexing agents, and thus demonstrates further the utility of the flavor-aversion conditioning paradigm in characterizing metal-chelator interactions.
条件性味觉厌恶范式被用于评估急性给予镉的毒性以及镉与重金属螯合剂二巯丙醇（BAL）和二巯基琥珀酸（DMSA）的相互作用。在摄入糖精后不久，大鼠腹腔注射单独的镉或与皮下注射BAL或DMSA联合使用的镉。三天后，让它们在饮用糖精或水之间进行选择，并记录对糖精的偏好。与未接受任何处理或接受赋形剂的大鼠相比，接受镉的大鼠对糖精的偏好显著降低（即条件性味觉厌恶）。单独给予BAL和DMSA时也能够产生条件性味觉厌恶。与单独接受镉的大鼠的味觉厌恶相比，接受镉与BAL或DMSA联合处理的大鼠表现出条件性味觉厌恶的显著但不完全的减弱。当BAL或DMSA的给药延迟4小时时，未获得螯合剂诱导的对镉诱导的味觉厌恶条件作用的阻断。BAL和DMSA对镉诱导的厌恶的减弱扩展了这些络合剂对铅诱导的味觉厌恶条件作用减弱的早期发现，从而进一步证明了味觉厌恶条件作用范式在表征金属-螯合剂相互作用中的效用。
