State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology (BUCT) , P.O. Box 37, Beijing 100029, China.
Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology (BUCT) , P.O. Box 37, Beijing 100029, China.
Langmuir. 2017 Jun 6;33(22):5577-5584. doi: 10.1021/acs.langmuir.7b00594. Epub 2017 May 25.
The use of the mixed catalytic system with several enzymes can provide multiple benefits in terms of the cost, simplification of a multistep reaction, and effectiveness of complex chemical reactions. Although study of different enzyme coimmobilization systems has attracted increasing attention in recent years, separately immobilizing enzymes which can not coexist on one support is still one of the great challenges. In this paper, a simple and effective strategy was introduced to separately encapsulate incompatible trypsin and transglutaminase (TGase) into different poly(ethylene glycol) (PEG) network layer grafted on low-density polyethylene (LDPE) film via visible light induced living photografting polymerization. As a proof of concept, this dual-enzyme separately loaded film was used to catalyze the synthesis of a new target antitumor drug LTV-azacytidine. The final results demonstrated that this strategy could maintain higher activities of both enzymes than the mixed coimmobilization method. And the mass spectra analysis results demonstrated that LTV-azacytidine was successfully synthesized. We believe that this facile and mild separately immobilizing incompatible enzyme strategy has great application potential in the field of biocatalysis.
使用混合催化体系中的几种酶可以在成本、多步反应的简化和复杂化学反应的有效性方面提供多种好处。尽管近年来对不同的酶共固定化体系的研究引起了越来越多的关注，但将不能共存于同一载体上的酶分别固定化仍然是一个巨大的挑战。在本文中，介绍了一种简单有效的策略，通过可见光诱导的活性光接枝聚合，将不相容的胰蛋白酶和转谷氨酰胺酶（TGase）分别包封到接枝在低密度聚乙烯（LDPE）膜上的不同聚乙二醇（PEG）网络层中。作为概念验证，该双酶分别负载的薄膜被用于催化新的抗肿瘤药物 LTV-氮杂胞苷的合成。最终结果表明，与混合共固定化方法相比，该策略可以保持两种酶的更高活性。并且质谱分析结果表明，LTV-氮杂胞苷已成功合成。我们相信，这种简便温和的分别固定化不相容酶的策略在生物催化领域具有巨大的应用潜力。
