State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China.
Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing, 100029, China.
Macromol Rapid Commun. 2018 Oct;39(20):e1800212. doi: 10.1002/marc.201800212. Epub 2018 Jun 26.
Entrapment of living cells into a polymer network has significant potential in various fields such as biomass conversion and tissue engineering. A crucial challenge for this strategy is to provide a mild enough condition to preserve cell viability. Here, a facile and cytocompatible method to entrap living yeast cells into a poly(ethylene glycol) (PEG) network grafting from polypropylene nonwoven fabrics via visible-light-induced surface living graft crosslinking polymerization is reported. Due to the mild reaction conditions and excellent biocompatibility of PEG, the immobilized yeast cells could maintain their viability and proliferate well. The obtained composite sheet has excellent long-term stability and shows no significant efficiency loss after 25 cycles of repeated batch bioethanol fermentation. The immobilized yeast cells exhibit 18.0% higher bioethanol fermentation efficiency than free cells. This strategy for immobilization of living cells with high viability has significant potential application.
将活细胞困在聚合物网络中在生物质转化和组织工程等领域具有重要的应用潜力。该策略的一个关键挑战是提供足够温和的条件来保持细胞的活力。本文报道了一种简便且细胞相容的方法，通过可见光诱导的表面活细胞接枝交联聚合，将活酵母细胞包埋在聚（乙二醇）（PEG）网络中，该网络接枝到丙纶无纺织物上。由于 PEG 的温和反应条件和优异的生物相容性，固定化酵母细胞能够保持其活力并很好地增殖。得到的复合片具有优异的长期稳定性，在重复批式生物乙醇发酵 25 个循环后没有明显的效率损失。固定化酵母细胞的生物乙醇发酵效率比游离细胞高 18.0%。这种具有高活力的活细胞固定化策略具有重要的潜在应用价值。
