Casey A L, Karpanen T J, Conway B R, Worthington T, Nightingale P, Waters R, Elliott T S J
Department of Clinical Microbiology, University Hospitals Birmingham NHS Foundation Trust, Edgbaston, B15 2WB, Birmingham, UK.
Department of Pharmacy, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, UK.
BMC Infect Dis. 2017 May 17;17(1):350. doi: 10.1186/s12879-017-2451-4.
Chlorhexidine (CHG) penetrates poorly into skin. The purpose of this study was to compare the depth of CHG skin permeation from solutions containing either 2% (w/v) CHG and 70% (v/v) isopropyl alcohol (IPA) or 2% (w/v) CHG, 70% (v/v) IPA and 2% (v/v) 1,8-cineole.
An ex-vivo study using Franz diffusion cells was carried out. Full thickness human skin was mounted onto the cells and a CHG solution, with or without 2% (v/v) 1,8-cineole was applied to the skin surface. After twenty-four hours the skin was sectioned horizontally in 100 μm slices to a depth of 2000 μm and the concentration of CHG in each section quantified using high performance liquid chromatography (HPLC). The data were analysed with repeated measures analysis of variance.
The concentration of CHG in the skin on average was significantly higher (33.3% [95%, CI 1.5% - 74.9%]) when a CHG solution which contained 1,8-cineole was applied to the skin compared to a CHG solution which did not contain this terpene (P = 0.042).
Enhanced delivery of CHG can be achieved in the presence of 1,8-cineole, which is the major component of eucalyptus oil. This may reduce the numbers of microorganisms located in the deeper layers of the skin which potentially could decrease the risk of surgical site infection.
洗必泰（CHG）对皮肤的渗透较差。本研究的目的是比较含有2%（w/v）CHG和70%（v/v）异丙醇（IPA）的溶液与含有2%（w/v）CHG、70%（v/v）IPA和2%（v/v）1,8-桉叶素的溶液中CHG的皮肤渗透深度。
采用Franz扩散池进行体外研究。将全层人体皮肤安装在扩散池上，在皮肤表面涂抹含或不含2%（v/v）1,8-桉叶素的CHG溶液。24小时后，将皮肤水平切成100μm厚的切片，深度达2000μm，使用高效液相色谱法（HPLC）对每个切片中的CHG浓度进行定量。数据采用重复测量方差分析。
与不含这种萜烯的CHG溶液相比，当向皮肤涂抹含有1,8-桉叶素的CHG溶液时，皮肤中CHG的平均浓度显著更高（33.3% [95%，置信区间1.5% - 74.9%]）（P = 0.042）。
在1,8-桉叶素（桉叶油的主要成分）存在的情况下，CHG的递送可得到增强。这可能会减少位于皮肤深层的微生物数量，从而有可能降低手术部位感染的风险。
