桉油增强洗必泰的皮肤渗透性。
Enhanced chlorhexidine skin penetration with eucalyptus oil.
机构信息
Life & Health Sciences, Aston University, Aston Triangle, Birmingham, UK.
出版信息
BMC Infect Dis. 2010 Sep 22;10:278. doi: 10.1186/1471-2334-10-278.
BACKGROUND
Chlorhexidine digluconate (CHG) is a widely used skin antiseptic, however it poorly penetrates the skin, limiting its efficacy against microorganisms residing beneath the surface layers of skin. The aim of the current study was to improve the delivery of chlorhexidine digluconate (CHG) when used as a skin antiseptic.
METHOD
Chlorhexidine was applied to the surface of donor skin and its penetration and retention under different conditions was evaluated. Skin penetration studies were performed on full-thickness donor human skin using a Franz diffusion cell system. Skin was exposed to 2% (w/v) CHG in various concentrations of eucalyptus oil (EO) and 70% (v/v) isopropyl alcohol (IPA). The concentration of CHG (μg/mg of skin) was determined to a skin depth of 1500 μm by high performance liquid chromatography (HPLC).
RESULTS
The 2% (w/v) CHG penetration into the lower layers of skin was significantly enhanced in the presence of EO. Ten percent (v/v) EO in combination with 2% (w/v) CHG in 70% (v/v) IPA significantly increased the amount of CHG which penetrated into the skin within 2 min.
CONCLUSION
The delivery of CHG into the epidermis and dermis can be enhanced by combination with EO, which in turn may improve biocide contact with additional microorganisms present in the skin, thereby enhancing antisepsis.
背景
葡萄糖酸氯己定（CHG）是一种广泛使用的皮肤消毒剂，但其在皮肤中的渗透性差，限制了其对皮肤表面以下微生物的功效。本研究旨在提高葡萄糖酸氯己定（CHG）作为皮肤消毒剂的使用效果。
方法
将氯己定涂于供体皮肤表面，并在不同条件下评估其渗透和保留情况。使用 Franz 扩散细胞系统对全厚供体人体皮肤进行皮肤渗透研究。皮肤暴露于不同浓度桉树油（EO）和 70%（v/v）异丙醇（IPA）中的 2%（w/v）CHG。通过高效液相色谱法（HPLC）测定 CHG 的浓度（μg/mg 皮肤）至 1500μm 的皮肤深度。
结果
在 EO 的存在下，2%（w/v）CHG 渗透到皮肤的下层的量明显增加。10%（v/v）EO 与 2%（w/v）CHG 在 70%（v/v）IPA 中的组合在 2 分钟内显著增加了渗透到皮肤中的 CHG 量。
结论
通过与 EO 结合，可以增强 CHG 进入表皮和真皮的输送，从而可以更好地接触皮肤中存在的其他微生物，提高消毒效果。
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