Gouveia-Figueira Sandra, Goldin Kristina, Hashemian Sanaz A, Lindberg Agneta, Persson Monica, Nording Malin L, Laurell Katarina, Fowler Christopher J
Department of Chemistry, Umeå University, SE-90187 Umeå, Sweden.
Department of Neurology, Östersund Hospital, SE-83183 Östersund, Sweden.
Prostaglandins Leukot Essent Fatty Acids. 2017 May;120:15-24. doi: 10.1016/j.plefa.2017.04.005. Epub 2017 Apr 21.
There is evidence that patients with migraine have deficient levels of the endogenous cannabinoid receptor ligand anandamide (AEA). It is not known, however, if this is a localised or generalised phenomenon. In the present study, levels of AEA, related N-acylethanolamines (NAEs) and linoleic acid-derived oxylipins have been measured in the blood of 26 healthy women and 38 women with migraine (26 with aura, 12 without aura) who were matched for age and body-mass index. Blood samples were taken on two occasions: the first sample near the start of the menstrual cycle (when present) and the second approximately fourteen days later. For a subset of migraine patients, two additional blood samples were taken, one during a migraine attack and one approximately 1 month later (to be at the same stage in the menstrual cycle, when present). NAEs and oxylipins were measured by liquid chromatography coupled to mass spectrometry. Twenty-nine lipids were quantified, of which 16 were found to have a high reproducibility of measurement. There were no significant differences in the levels of AEA, the related NAEs stearoylethanolamide and oleoylethanolamide or any of the nine linoleic acid-derived oxylipins measured either between migraine patients with vs. without aura, or between controls and migraine patients (after stratification to take into account whether or not the individuals had regular menstruation cycles) in either of the first two samples. Levels of linoleoylethanolamide were lower in the patients with vs. without aura on the second sample but not in the first sample, but the biological importance of this finding is unclear. Due to time-dependent increases in their concentrations ex vivo prior to centrifugation, AEA and oleoylethanolamide levels in the samples collected during migraine attacks were not analysed, but for the other fourteen lipids, there were no significant differences in plasma concentrations during migraine vs. one month later. It is concluded that migraine is not associated with a generalised (as opposed to localised) deficiency in these lipids.
有证据表明，偏头痛患者体内内源性大麻素受体配体花生四烯酸乙醇胺（AEA）水平不足。然而，尚不清楚这是局部现象还是全身性现象。在本研究中，测量了26名健康女性和38名偏头痛女性（26名有先兆，12名无先兆）血液中AEA、相关的N-酰基乙醇胺（NAEs）和亚油酸衍生的氧化脂质水平，这些女性在年龄和体重指数方面相匹配。在两个时间点采集血样：第一个样本在月经周期开始时（如果有月经），第二个样本大约在十四天后。对于一部分偏头痛患者，额外采集了两个血样，一个在偏头痛发作期间，另一个大约在1个月后（处于月经周期的同一阶段，如果有月经）。通过液相色谱-质谱联用技术测量NAEs和氧化脂质。对29种脂质进行了定量分析，其中16种脂质的测量具有较高的重现性。在前两个样本中，无论是有先兆还是无先兆的偏头痛患者之间，或者是对照组与偏头痛患者之间（在分层考虑个体是否有规律月经周期后），所测量的AEA、相关的NAEs硬脂酰乙醇胺和油酰乙醇胺水平，以及九种亚油酸衍生的氧化脂质中的任何一种水平，均无显著差异。在第二个样本中，但不是在第一个样本中，有先兆的患者中亚油酰乙醇胺水平低于无先兆的患者，但其生物学意义尚不清楚。由于离心前样本中AEA和油酰乙醇胺浓度会随时间增加，因此未分析偏头痛发作期间采集的样本中的AEA和油酰乙醇胺水平，但对于其他14种脂质，偏头痛期间与1个月后的血浆浓度无显著差异。结论是，偏头痛与这些脂质的全身性（而非局部性）缺乏无关。
