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Enhancement of the apparent cleavage specificities of restriction endonucleases: applications to megabase mapping of chromosomes.

作者信息

McClelland M, Nelson M

机构信息

Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.

出版信息

Gene Amplif Anal. 1987;5:257-82.

PMID:2851534
Abstract

We have described how DNA methylases may be used to enhance the apparent specificities of restriction endonucleases [9-11, 17, 18] to generate DNA fragments averaging 6000 to 270,000,000 base pairs on random DNA. Taking into account the non-random arrangement of natural DNA, we have predicted the rarity of certain recognition sequences in the genomes of several species.

摘要

相似文献

1
Enhancement of the apparent cleavage specificities of restriction endonucleases: applications to megabase mapping of chromosomes.
Gene Amplif Anal. 1987;5:257-82.
2
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引用本文的文献

1
Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.位点特异性修饰对限制性核酸内切酶和DNA修饰甲基转移酶的影响。
Nucleic Acids Res. 1994 Sep;22(17):3640-59. doi: 10.1093/nar/22.17.3640.
2
Enzymatic cleavage of a bacterial genome at a 10-base-pair recognition site.在一个10个碱基对识别位点对细菌基因组进行酶切。
Proc Natl Acad Sci U S A. 1989 Jan;86(1):51-5. doi: 10.1073/pnas.86.1.51.
3
Cleavage at the twelve-base-pair sequence 5'-TCTAGATCTAGA-3' using M.Xbal (TCTAGm6A) methylation and DpnI (Gm6A/TC) cleavage.
Nucleic Acids Res. 1990 Mar 25;18(6):1603-7. doi: 10.1093/nar/18.6.1603.
4
Enzymatic cleavage of a bacterial chromosome at a transposon-inserted rare site.在转座子插入的稀有位点对细菌染色体进行酶切。
Nucleic Acids Res. 1991 Feb 25;19(4):829-32. doi: 10.1093/nar/19.4.829.
5
Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化：对DNA修饰甲基转移酶和限制性内切酶的影响。
Nucleic Acids Res. 1991 Apr 25;19 Suppl(Suppl):2045-71. doi: 10.1093/nar/19.suppl.2045.
6
Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化对DNA修饰甲基转移酶和限制性内切酶的影响。
Nucleic Acids Res. 1992 May 11;20 Suppl(Suppl):2145-57. doi: 10.1093/nar/20.suppl.2145.
7
Dissection of the Salmonella typhimurium genome by use of a Tn5 derivative carrying rare restriction sites.利用携带稀有限制性酶切位点的Tn5衍生物对鼠伤寒沙门氏菌基因组进行剖析。
J Bacteriol. 1992 Jun;174(11):3807-11. doi: 10.1128/jb.174.11.3807-3811.1992.