Pandurangan Muthuraman, Enkhtaivan Gansukh, Mistry Bhupendra, Patel Rahul V, Moon Sohyun, Kim Doo Hwan
Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
Department of Food Science and Biotechnology, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 410-820, South Korea.
Amino Acids. 2017 Aug;49(8):1373-1380. doi: 10.1007/s00726-017-2437-y. Epub 2017 May 17.
β-Alanine is a non-essential amino acid and presents as a major component of various sports supplements. It is a non-proteogenic amino acid, formed in vivo by degradation of carnosine, anserine, balenine, and dihydrouracil. The present study was aimed at investigating the anti-tumor effects of β-alanine in renal and cervical tumor cells. Sulforhodamine-B assay and flow cytometric analysis were used to measure cell viability. Lactate dehydrogenase (LDH) expression was analyzed using FITC-conjugated fluorescent antibody. The cellular adenosine triphosphate (ATP) content was measured using bioluminescence method. Cell migration was determined by the simple standard-scratch method. β-Alanine reduced renal and cervical cell growth significantly. Percentage of inhibition of renal and cervical tumor cells was increased at higher concentration of β-alanine. LDH expression and ATP content were significantly reduced in renal and cervical tumor cells in a dose-dependent manner. Renal and cervical tumor cell migration were significantly reduced following 10 and 100 mM of β-alanine treatment. In our study, β-alanine exerts no significant effect on normal MDCK cells except a marginal effect at the highest concentration (100 mM). In summary, our experimental data suggest that β-alanine may be a potential anti-tumor agent exhibiting several anti-cancer effects in renal and cervical tumor cells.
β-丙氨酸是一种非必需氨基酸，是各种运动补剂的主要成分。它是一种非蛋白质氨基酸，在体内由肌肽、鹅肌肽、鲸肌肽和二氢尿嘧啶降解形成。本研究旨在探讨β-丙氨酸对肾肿瘤细胞和宫颈肿瘤细胞的抗肿瘤作用。采用磺酰罗丹明-B法和流式细胞术分析来测定细胞活力。使用异硫氰酸荧光素（FITC）偶联的荧光抗体分析乳酸脱氢酶（LDH）表达。采用生物发光法测定细胞内三磷酸腺苷（ATP）含量。通过简单的标准划痕法测定细胞迁移。β-丙氨酸显著降低肾肿瘤细胞和宫颈肿瘤细胞的生长。在较高浓度的β-丙氨酸作用下，肾肿瘤细胞和宫颈肿瘤细胞的抑制率增加。肾肿瘤细胞和宫颈肿瘤细胞中的LDH表达及ATP含量呈剂量依赖性显著降低。在10 mM和100 mM的β-丙氨酸处理后，肾肿瘤细胞和宫颈肿瘤细胞的迁移显著减少。在我们的研究中，β-丙氨酸对正常的犬肾细胞（MDCK细胞）无显著影响，仅在最高浓度（100 mM）时有轻微影响。总之，我们的实验数据表明，β-丙氨酸可能是一种潜在的抗肿瘤药物，对肾肿瘤细胞和宫颈肿瘤细胞具有多种抗癌作用。
