Sun Xin, Wang Shengyu, Lin Xian, Zhao Lianzhong, Zhang Dan, Yi Chenyang, Sun Xiaomei, Chen Huanchun, Jin Meilin
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, P. R. China.
Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. China.
Proteomics. 2017 Jun;17(12). doi: 10.1002/pmic.201700033.
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused huge economic losses to the duck industry in China since 2010. Moreover, the infection has spread rapidly, posing a potential public health concern. In this study, iTRAQ approach was first used to quantitatively identify differentially expressed cellular proteins in DTMUV-infected BHK-21 cells which are usually employed to produce veterinary vaccines for DTMUV, as well as other flaviviruses by serial passage. We identified 192 differentially expressed cellular proteins, including 11 upregulated and eight downregulated proteins at 24 h postinfection (hpi), as well as 25 upregulated and 151 downregulated proteins at 48 hpi, of which TLR9, DDX3X, and DDX5 may play important roles in virus propagation. Further, DDX3X could inhibit DTMUV replication by modulating the IFN pathway via TBK1. In conclusion, our study is the first to analyze the protein profile of DTMUV-infected cells by quantitative proteomics. We believe that our findings provide valuable information in better understanding the host response to DTMUV infection. These findings are particularly important in the development of vaccine-based strategies.
鸭坦布苏病毒（DTMUV）是一种新出现的致病性黄病毒，自2010年以来给中国鸭业造成了巨大经济损失。此外，该病毒感染传播迅速，对公共卫生构成潜在威胁。在本研究中，首次采用iTRAQ技术定量鉴定DTMUV感染的BHK-21细胞中差异表达的细胞蛋白，BHK-21细胞通常用于通过连续传代生产针对DTMUV以及其他黄病毒的兽用疫苗。我们鉴定出192种差异表达的细胞蛋白，包括感染后24小时（hpi）时11种上调和8种下调的蛋白，以及48 hpi时25种上调和151种下调的蛋白，其中TLR9、DDX3X和DDX5可能在病毒增殖中发挥重要作用。此外，DDX3X可通过TBK1调节IFN通路来抑制DTMUV复制。总之，我们的研究首次通过定量蛋白质组学分析了DTMUV感染细胞的蛋白质谱。我们相信，我们的发现为更好地理解宿主对DTMUV感染的反应提供了有价值的信息。这些发现在基于疫苗的策略开发中尤为重要。
