Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Sci Rep. 2018 Jul 4;8(1):10079. doi: 10.1038/s41598-018-28499-3.
Duck reovirus (DRV) is an typical aquatic bird pathogen belonging to the Orthoreovirus genus of the Reoviridae family. Reovirus causes huge economic losses to the duck industry. Although DRV has been identified and isolated long ago, the responses of Cairna moschata to classical/novel duck reovirus (CDRV/NDRV) infections are largely unknown. To investigate the relationship of pathogenesis and immune response, proteomes of C. moschata liver cells under the C/NDRV infections were analyzed, respectively. In total, 5571 proteins were identified, among which 5015 proteins were quantified. The differential expressed proteins (DEPs) between the control and infected liver cells displayed diverse biological functions and subcellular localizations. Among the DEPs, most of the metabolism-related proteins were down-regulated, suggesting a decrease in the basal metabolisms under C/NDRV infections. Several important factors in the complement, coagulation and fibrinolytic systems were significantly up-regulated by the C/NDRV infections, indicating that the serine protease-mediated innate immune system might play roles in the responses to the C/NDRV infections. Moreover, a number of molecular chaperones were identified, and no significantly changes in their abundances were observed in the liver cells. Our data may give a comprehensive resource for investigating the regulation mechanism involved in the responses of C. moschata to the C/NDRV infections.
鸭呼肠孤病毒（DRV）是一种典型的水生鸟类病原体，属于呼肠孤病毒科的正呼肠孤病毒属。呼肠孤病毒给养鸭业造成了巨大的经济损失。尽管 DRV 早已被鉴定和分离，但麝香鸭对经典/新型鸭呼肠孤病毒（CDRV/NDRV）感染的反应在很大程度上是未知的。为了研究发病机制和免疫反应的关系，分别分析了麝香鸭肝细胞在 C/NDRV 感染下的蛋白质组。总共鉴定到 5571 种蛋白质，其中 5015 种蛋白质被定量。对照和感染的肝细胞之间差异表达的蛋白质（DEPs）显示出不同的生物学功能和亚细胞定位。在 DEPs 中，大多数与代谢相关的蛋白质下调，表明在 C/NDRV 感染下基础代谢降低。补体、凝血和纤溶系统中的几个重要因子被 C/NDRV 感染显著上调，表明丝氨酸蛋白酶介导的先天免疫系统可能在对 C/NDRV 感染的反应中发挥作用。此外，还鉴定到了许多分子伴侣，但在肝细胞中它们的丰度没有明显变化。我们的数据可能为研究麝香鸭对 C/NDRV 感染的反应中涉及的调节机制提供全面的资源。
