Department of Chemistry, Virginia Polytechnic Institute and State University , Blacksburg, Virginia 24061, United States.
Department of Orthopaedic Surgery, University of Virginia , Charlottesville, Virginia 22903, United States.
ACS Appl Mater Interfaces. 2017 May 31;9(21):17681-17687. doi: 10.1021/acsami.7b04718. Epub 2017 May 19.
Antioxidative and anti-inflammatory effects of trimetallic nitride endohedral fullerenes carboxyl-GdN@C, a newly developed magnetic resonance imaging (MRI) contrast agent, were investigated. All hydrochalarone and carboxyl-functionalized fullerenes showed effective radical (hydroxyl and superoxide anion) scavenging, whereas the carboxyl-GdN@C more efficiently attenuated lipopolysaccharide (LPS) induced oxidative stress in macrophages. Carboxyl-GdN@C also suppressed LPS-elicited mRNA expression of pro-inflammatory inducible nitric oxide synthase and tumor necrosis factor-alpha, and upregulated antioxidative enzyme axis Nrf2 and heme oxygenase-1, possibly via ERK but not AKT signaling pathways. Therefore, carboxyl-GdN@C held a great promise in becoming a novel theranostic nanoplatform for simultaneously deliver MRI contrast and therapeutic functions to inflammation-related diseases.
研究了新型磁共振成像（MRI）造影剂三金属氮化物笼状富勒烯羧基-GdN@C 的抗氧化和抗炎作用。所有的氢氯沙隆和羧基功能化富勒烯都表现出有效的自由基（羟基和超氧阴离子）清除作用，而羧基-GdN@C 则更有效地减轻了巨噬细胞中脂多糖（LPS）诱导的氧化应激。羧基-GdN@C 还抑制了 LPS 诱导的促炎诱导型一氧化氮合酶和肿瘤坏死因子-α的 mRNA 表达，并上调了抗氧化酶轴 Nrf2 和血红素加氧酶-1，可能通过 ERK 而不是 AKT 信号通路。因此，羧基-GdN@C 有望成为一种新型的治疗诊断纳米平台，可同时将 MRI 对比剂和治疗功能递送到与炎症相关的疾病。
