Ring Christine M, Iqbal Emil S, Hacker David E, Hartman Matthew C T, Cropp T Ashton
Virginia Commonwealth University, Department of Chemistry, 1001 West Main Street, Richmond, Virginia 23284-2006, USA.
Org Biomol Chem. 2017 May 31;15(21):4536-4539. doi: 10.1039/c7ob00844a.
Due to the lowered pK of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures.
由于4-氟组氨酸的pK相对于组氨酸降低，含有这种氨基酸的肽和蛋白质可能具有新的特性。我们在此报告4-氟组氨酸的优化合成，并表明它可以在体外翻译反应中有效替代组氨酸。此外，在存在组氨酸标签蛋白混合物的情况下，含有6×-氟组氨酸标签的肽能够从镍树脂中被选择性捕获和洗脱。
