Zhu Y, Zhang X, Wu X, Chen G, Bakken L R, Zhao L, Frostegård Å, Zhang X
State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Department of Environmental Science, Norwegian University of Life Sciences, Ås, Norway.
Lett Appl Microbiol. 2017 Aug;65(2):159-164. doi: 10.1111/lam.12756. Epub 2017 Jul 4.
Bacteria of Dechloromonas were recognized as potential functional important denitrifiers in a long-term shell sand-amended peat soil. Different microcosms in a solid matrix and slurry systems with the addition of carbon and nitrogen sources, for example, clover leaves, glutamate and nitrate, were established. The bacterial community structures were analysed by pyrosequencing of the 16S rRNA gene to select the conditions for enriching bacteria of Dechloromonas. The results showed that a relatively even bacterial community in the initial soil shifted to communities dominated by a few types of nitrate-reducing bacteria after the incubation, which strongly responded to the carbon substrates addition and consumption. The bacteria of several genera including Dechloromonas, Pseudomonas, Clostridium, Aeromonas and Ferribacterium were significantly enriched after a certain period of time. The bacteria of Dechloromonas became one of the most predominant bacteria in the incubated community. Especially when added the mixed carbon substrates into the solid soil matrix, as high as 34% of abundance was detected. This study proved that the functional important bacteria from the genus of Dechloromonas could be enriched to an extremely high abundance by using proper culture condition which will benefit to the isolation or direct metagenomics study for Dechloromonas.
The study of key players in a microbial community is always of important. In this study, the functional important denitrifiers in a shell sand-amended peat soil were investigated. Using different carbon sources in the incubation, we found the bacteria from the genus of Dechloromonas were enriched to an abundance of higher than 34% with several other denitrifiers together. This work provides us helpful insights not only for knowing the diversity of denitrifiers in the studied peat soil, but also for understanding their response to the carbon sources and the culture conditions.
在长期添加贝壳砂的泥炭土中，脱氯单胞菌属细菌被认为是具有潜在功能重要性的反硝化菌。建立了在固体基质和泥浆系统中添加碳源和氮源（如三叶草叶、谷氨酸和硝酸盐）的不同微观世界。通过对16S rRNA基因进行焦磷酸测序分析细菌群落结构，以选择富集脱氯单胞菌属细菌的条件。结果表明，初始土壤中相对均匀的细菌群落经过培养后转变为由几种类型的硝酸盐还原菌主导的群落，这对碳底物的添加和消耗有强烈反应。包括脱氯单胞菌属、假单胞菌属、梭菌属、气单胞菌属和铁杆菌属在内的几个属的细菌在一段时间后显著富集。脱氯单胞菌属细菌成为培养群落中最主要的细菌之一。特别是当将混合碳底物添加到固体土壤基质中时，检测到的丰度高达34%。本研究证明，通过使用合适的培养条件，可以将具有功能重要性的脱氯单胞菌属细菌富集到极高的丰度，这将有利于脱氯单胞菌的分离或直接宏基因组学研究。
对微生物群落中关键参与者的研究一直很重要。在本研究中，对添加贝壳砂的泥炭土中具有功能重要性的反硝化菌进行了研究。在培养过程中使用不同的碳源，我们发现脱氯单胞菌属细菌与其他几种反硝化菌一起富集到高于34%的丰度。这项工作不仅为了解所研究泥炭土中反硝化菌的多样性提供了有益的见解，也为理解它们对碳源和培养条件的反应提供了帮助。
