Shelton K R, Egle P M, Todd J M
Biochem Biophys Res Commun. 1986 Jan 29;134(2):492-8. doi: 10.1016/s0006-291x(86)80447-8.
A step in the induction of a 30- to 35-kD stress protein may be the reaction of chemical inducers with glutathione. Effective inducers are sulfhydryl reagents. Further, a comparison of three reagents, 1-chloro-2,4-dinitrobenzene, diethylmaleate, and N-ethylmaleimide, indicates that the first two, which have considerable selectivity for glutathione, are strong inducers of the stress protein but the third, which is much more reactive with protein sulfhydryls, is either a poor or ineffective inducer. A decrease in cellular glutathione does not appear to be inductive, however. An increase in modified glutathione remains as a possible signal for the induction of this stress protein.
诱导产生30至35千道尔顿应激蛋白的一个步骤可能是化学诱导剂与谷胱甘肽的反应。有效的诱导剂是巯基试剂。此外，对三种试剂1-氯-2,4-二硝基苯、顺丁烯二酸二乙酯和N-乙基马来酰亚胺的比较表明，前两种试剂对谷胱甘肽具有相当高的选择性，是应激蛋白的强诱导剂，但第三种试剂与蛋白质巯基的反应性更强，要么是弱诱导剂，要么是无效诱导剂。然而，细胞内谷胱甘肽的减少似乎没有诱导作用。修饰型谷胱甘肽的增加仍是诱导这种应激蛋白的一个可能信号。
