LeFurgey A, Ingram P, Lieberman M
Department of Cell Biology, Duke University School of Medicine, Durham, North Carolina.
Cell Calcium. 1988 Dec;9(5-6):219-35. doi: 10.1016/0143-4160(88)90003-6.
Quantitative electron probe X-ray imaging techniques have been utilized to determine simultaneously the element content within a single cultured embryonic chick heart cell and its intracellular compartments as well as the average elemental content of several heart cells within a population. These features of microchemical imaging have permitted establishment of data regarding: (1) the heterogeneity of calcium accumulation in mitochondrial, cytoplasmic and nuclear compartments under conditions which elevate total cell calcium without producing irreversible cell injury; and (2) the variability of calcium accumulation from cell to cell within the population sampled. The results indicate that during Na-K pump inhibition (K-free HT-BSS, 10(-4) M ouabain, 60 min) elevation of mitochondrial calcium, measured in situ by electron probe X-ray microanalysis, to levels more than 100 times greater than in the basal state, may not cause irreversible mitochondrial uncoupling and cell death.
定量电子探针X射线成像技术已被用于同时测定单个培养的胚胎鸡心脏细胞及其细胞内区室中的元素含量，以及群体中几个心脏细胞的平均元素含量。这种微化学成像的特点使得能够建立关于以下方面的数据：(1) 在不产生不可逆细胞损伤的情况下提高细胞总钙含量的条件下，线粒体、细胞质和细胞核区室中钙积累的异质性；(2) 所采样群体中细胞间钙积累的变异性。结果表明，在钠钾泵抑制期间（无钾的HT-BSS、10(-4) M哇巴因，60分钟），通过电子探针X射线微量分析原位测量的线粒体钙升高至比基础状态高100倍以上的水平，可能不会导致不可逆的线粒体解偶联和细胞死亡。
