Cariou R, Harousseau J L, Tobelem G
Service d'hématologie clinique, CHU Nantes, France.
Cell Biol Int Rep. 1988 Dec;12(12):1037-47. doi: 10.1016/0309-1651(88)90028-8.
Previous works have reported the controversial effects of heparin and steroids on angiogenesis. In this study, we investigated the effect of these compounds on human endothelial cell (EC) growth in vitro. An antiproliferative heparin activity was found in low human serum concentrations (2%). When EC were exposed to heparin (10(-6) M), their proliferation index was reduced in the presence of endothelial cell growth factor added 6 hours or more later. These results suggest that there is an intracellular effect of heparin which reduces 3H-methylthymidine uptake. Hydrocortisone acetate and tetrahydroS induced inhibition of EC growth in a dose-dependent manner. Steroids inhibited proliferation of EC in culture medium in the presence or the absence of growth factor and in different human serum concentrations. These results suggest a possible synergistic antiangiogenic action of heparin plus steroids.
先前的研究报道了肝素和类固醇对血管生成的争议性作用。在本研究中，我们调查了这些化合物对人内皮细胞（EC）体外生长的影响。在低浓度人血清（2%）中发现了肝素的抗增殖活性。当EC暴露于肝素（10⁻⁶ M）时，在6小时或更长时间后添加内皮细胞生长因子的情况下，其增殖指数降低。这些结果表明肝素存在细胞内效应，可减少³H-甲基胸腺嘧啶核苷的摄取。醋酸氢化可的松和四氢S以剂量依赖的方式诱导EC生长抑制。类固醇在有或无生长因子以及不同人血清浓度的培养基中均抑制EC的增殖。这些结果提示肝素加类固醇可能具有协同抗血管生成作用。
