Mineo C, Kanaseki T, Enomoto M, Ohkuma S, Takano T
Department of Microbiology and Molecular Pathology, Teikyo University, Kanagawa, Japan.
Cell Struct Funct. 1988 Oct;13(5):435-43. doi: 10.1247/csf.13.435.
The mechanism through which nonmembranous lipid inclusion bodies consisting of cholesteryl esters accumulate in the cytoplasm was studied. Most lipid inclusion bodies in macrophages after 24 h incubation with anisotropic cholesteryl oleate liquid crystals were surrounded by a limiting membrane. The limiting membrane, however, could not be observed after further incubation for 48 h in the presence of esterastin, which is known to be an inhibitor of lipase and esterase. Under these conditions, the levels of hydrolysis and re-esterification of cholesteryl esters were less than 15% and 5% of the control ones, respectively. These results suggest that the inclusion bodies were transferred from lysosomes to the cytoplasm, with partial hydrolysis of cholesteryl esters, in addition to through the pathway via microsomes.
研究了由胆固醇酯组成的非膜性脂质包涵体在细胞质中积累的机制。用各向异性油酸胆固醇液晶孵育24小时后，巨噬细胞中的大多数脂质包涵体被一层界膜包围。然而，在已知为脂肪酶和酯酶抑制剂的酯抑素存在下进一步孵育48小时后，无法观察到界膜。在这些条件下，胆固醇酯的水解和再酯化水平分别低于对照的15%和5%。这些结果表明，除了通过微粒体途径外，包涵体还通过胆固醇酯的部分水解从溶酶体转移到细胞质中。
