老年女性居家间歇步行训练期间奶制品摄入对大腿肌肉力量和NFKB基因甲基化的影响：一项随机对照试验性研究
Effects of milk product intake on thigh muscle strength and NFKB gene methylation during home-based interval walking training in older women: A randomized, controlled pilot study.
作者信息
Masuki Shizue, Nishida Kensei, Hashimoto Shigenari, Morikawa Mayuko, Takasugi Satoshi, Nagata Masashi, Taniguchi Shun'ichiro, Rokutan Kazuhito, Nose Hiroshi
机构信息
Department of Sports Medical Sciences, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
Institute for Biomedical Sciences, Shinshu University, Matsumoto, Japan.
出版信息
PLoS One. 2017 May 17;12(5):e0176757. doi: 10.1371/journal.pone.0176757. eCollection 2017.
BACKGROUND
Muscle atrophy with aging is closely associated with chronic systemic inflammation and lifestyle-related diseases. In the present study, we assessed whether post-exercise milk product intake during 5-month interval walking training (IWT) enhanced the increase in thigh muscle strength and ameliorated susceptibility to inflammation in older women.
METHODS
Subjects [n = 37, 66±5 (standard deviation) yrs] who had been performing IWT for >6 months participated in this study. They were randomly divided into the following 3 groups: IWT alone (CNT, n = 12), IWT + low-dose post-exercise milk product intake (LD, n = 12; 4 g protein and 3 g carbohydrate) or IWT + a 3-times higher dose of milk product intake than the LD group (HD, n = 13). They were instructed to repeat ≥5 sets of fast and slow walking for 3 min each at ≥70% and 40% peak aerobic capacity for walking, respectively, per day for ≥4 days/week.
RESULTS
After IWT, thigh muscle strength increased in the HD group (8±2%) more than in the CNT group (-2±3%, P = 0.022), despite similar IWT achievements between the groups (P>0.15). Pyrosequencing analysis using whole blood showed that methylation of NFKB1 and NFKB2, master genes of inflammation, was enhanced in the HD group (29±7% and 44±11%, respectively) more than in the CNT group (-20±6% and -10±6%, respectively; P<0.001). Moreover, the genome-wide DNA methylation analysis showed that several inflammation-related genes were hyper-methylated in the HD group compared with that in the CNT group, suggesting greater pro-inflammatory cytokine gene suppression in the HD group.
CONCLUSION
HD milk product intake after exercise produced a greater percent increase in thigh muscle strength and NFKB1 and NFKB2 gene methylation during IWT in physically active older women.
TRIAL REGISTRATION
UMIN-CTR No. UMIN000024544 and No. UMIN000024912.
背景
随着年龄增长出现的肌肉萎缩与慢性全身炎症及生活方式相关疾病密切相关。在本研究中，我们评估了在为期5个月的间歇步行训练（IWT）期间运动后摄入奶制品是否能增强老年女性大腿肌肉力量的增加并改善炎症易感性。
方法
进行IWT超过6个月的受试者[n = 37，66±5（标准差）岁]参与了本研究。他们被随机分为以下3组：仅进行IWT（对照组，n = 12）、IWT +低剂量运动后奶制品摄入（低剂量组，n = 12；4克蛋白质和3克碳水化合物）或IWT +比低剂量组高3倍的奶制品摄入量（高剂量组，n = 13）。他们被要求每周≥4天，每天分别以≥70%和40%的步行峰值有氧能力重复进行≥5组快走和慢走，每组3分钟。
结果
IWT后，高剂量组大腿肌肉力量增加（8±2%），高于对照组（-2±3%，P = 0.022），尽管两组的IWT成绩相似（P>0.15）。使用全血的焦磷酸测序分析表明，炎症主基因NFKB1和NFKB2的甲基化在高剂量组中增强（分别为29±7%和44±11%），高于对照组（分别为-20±6%和-10±6%；P<0.001）。此外，全基因组DNA甲基化分析表明，与对照组相比，高剂量组中几个炎症相关基因发生了高甲基化，表明高剂量组中促炎细胞因子基因的抑制作用更强。
结论
在身体活跃的老年女性中，运动后摄入高剂量奶制品在IWT期间使大腿肌肉力量以及NFKB1和NFKB2基因甲基化的增加百分比更大。
试验注册
UMIN-CTR编号UMIN000024544和UMIN000024912。
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