Fukushima H, Ueno S, Sakakida M, Takahashi T, Shirotani T, Araki E, Kishikawa H, Yamaguchi K, Nakamura N, Uzawa H
Department of Metabolic Medicine, Kumamoto University Medical School, Japan.
Nihon Naibunpi Gakkai Zasshi. 1988 Aug 20;64(8):666-76. doi: 10.1507/endocrine1927.64.8_666.
Acute effects of somatostatin analog (SMS 201-995) on pancreatic hormones were studied in two patients with malignant islet-cell carcinoma. Before and after subcutaneous injection of somatostatin with a doses of 50 micrograms, blood glucose (BG), serum growth hormone (hGH), C-peptide immunoreactivity (CPR), plasma immunoreactive glucagon (IRG) and gastrin were assayed, and changes in elution patterns of IRG and gastrin were also analyzed on Bio-Gel P-30 column chromatography. In Patient 1 with glucagonoma syndrome and hypergastrinemia, a prompt and remarkable decrease in plasma IRG and gastrin was observed after the injection of SMS 201-995 in association with a decrease in blood glucose, and then IRG and gastrin increased gradually. The suppressive effect continued for at least 6 hours. On gel filtration of the plasma obtained before the injection of the analog, three major peaks, greater than 20000, 9000 and 3500 molecular-weight (mol wt) fractions, were seen in IRG fraction. The decrease in plasma IRG observed at 1 hour after the injection was mainly due to a marked decrease in the 3500 molecular weight fraction. In addition, a slight decrease in the 9000 mol wt fraction was seen. At 4 hours after the injection, the 3500 mol wt peak returned to the previous level, while the 9000 mol wt peak decreased further. On the other hand, the gastrin elution pattern of plasma obtained before the injection revealed three major gastrin peaks, greater than 20000, 7000 and 5000 mol wt fraction. The changes in the gastrin elution pattern after the injection were similar to those of the IRG elution pattern. In Patient 2 with Zollinger-Ellison's syndrome, the plasma gastrin level decreased gradually for 5 hours after the injection. On gel filtration of the plasma obtained before the injection, two major gastrin peaks, 7000 and 5000 mol wt fraction, of which the large-molecular fraction was more prominent than the small-molecular fraction, were observed. After the injection, a marked decrease in the small-molecular fraction and a gradual decrease in the large-molecular fraction were observed for 4 hours, accompanied by a decrease in plasma gastrin. At 7 hours after the injection, the smaller fraction was augmented again. The serum CPR and hGH was slightly suppressed after the injection in both patients. The adverse effects of slight nausea and vomiting were noticed only in Patient 1.(ABSTRACT TRUNCATED AT 400 WORDS)
在两名恶性胰岛细胞瘤患者中研究了生长抑素类似物（SMS 201-995）对胰腺激素的急性作用。皮下注射50微克生长抑素前后，测定血糖（BG）、血清生长激素（hGH）、C肽免疫反应性（CPR）、血浆免疫反应性胰高血糖素（IRG）和胃泌素，并在Bio-Gel P-30柱色谱上分析IRG和胃泌素洗脱模式的变化。在患有胰高血糖素瘤综合征和高胃泌素血症的患者1中，注射SMS 201-995后，血浆IRG和胃泌素迅速显著下降，同时血糖降低，然后IRG和胃泌素逐渐升高。抑制作用持续至少6小时。在注射类似物前获得的血浆进行凝胶过滤时，IRG组分中可见三个主要峰，分子量大于20000、9000和3500道尔顿（mol wt）组分。注射后1小时观察到的血浆IRG下降主要是由于3500分子量组分显著下降。此外，9000 mol wt组分略有下降。注射后4小时，3500 mol wt峰恢复到先前水平，而9000 mol wt峰进一步下降。另一方面，注射前获得的血浆胃泌素洗脱模式显示三个主要胃泌素峰，分子量大于20000、7000和5000 mol wt组分。注射后胃泌素洗脱模式的变化与IRG洗脱模式的变化相似。在患有卓-艾综合征的患者2中，注射后血浆胃泌素水平在5小时内逐渐下降。在注射前获得的血浆进行凝胶过滤时，观察到两个主要胃泌素峰，7000和5000 mol wt组分，其中大分子组分比小分子组分更突出。注射后，小分子组分显著下降，大分子组分在4小时内逐渐下降，同时血浆胃泌素下降。注射后7小时，较小的组分再次增加。两名患者注射后血清CPR和hGH均略有抑制。仅在患者1中注意到轻微恶心和呕吐的不良反应。（摘要截断于400字）
