Fukushima H, Yamaguchi K, Uzawa H
Endocrinol Jpn. 1981 Oct;28(5):575-82. doi: 10.1507/endocrj1954.28.575.
The heterogeneity of glucagon and insulin in plasma and tissue extracts from a 57-year-old female with glucagonoma syndrome with surgically and autopsy verified islet-cell tumors was studied by Bio-Gel P-10 filtration. The preoperative plasma immunoreactive glucagon (IRG) level was 20.2 ng/ml, and plasma glucagon-like immunoreactivity(GLI) 25.8 ng/ml. The column chromatography of the preoperative plasma revealed three or four IRG components and four GLI components. Among these, peak II, the large glucagon immunoreactivity (LGI) peak, considered a candidate for proglucagon, was prominent, along with peak III. The resected metastatic liver tumor contained an enormous amount of IRG and an appreciable amount of immunoreactive insulin (IRI), indicating that the elevated plasma IRG was mainly of tumor origin. The IRG pattern of the tumor tissue extract revealed a small quantity of IRG in peaks I and II, and a large amount in peak III; control pancreatic tissue extract manifested a similar elution pattern. The IRI elution pattern of the tumor tissue extract revealed two major IRI peaks which migrated close to the elution volume of cytochrome C and insulin, respectively. This is a quite different pattern from the control pancreatic tissue extract in which the RI peak was localized in the elution volume of the insulin. We conclude that the present metastatic liver tumor produced not only enormous amounts of glucagon but heterogeneous peptides which contained immunological insulin determinants within their.
采用生物凝胶P - 10过滤法，研究了一名57岁患有胰高血糖素瘤综合征且经手术及尸检证实存在胰岛细胞瘤的女性患者血浆和组织提取物中胰高血糖素和胰岛素的异质性。术前血浆免疫反应性胰高血糖素（IRG）水平为20.2 ng/ml，血浆胰高血糖素样免疫反应性（GLI）为25.8 ng/ml。术前血浆的柱色谱分析显示有三或四种IRG成分和四种GLI成分。其中，被认为是胰高血糖素原候选物的大胰高血糖素免疫反应性（LGI）峰即峰II，与峰III一样显著。切除的转移性肝肿瘤含有大量IRG和相当数量的免疫反应性胰岛素（IRI），这表明血浆IRG升高主要源于肿瘤。肿瘤组织提取物的IRG模式显示，峰I和峰II中有少量IRG，峰III中有大量IRG；对照胰腺组织提取物表现出类似的洗脱模式。肿瘤组织提取物的IRI洗脱模式显示有两个主要的IRI峰，分别接近细胞色素C和胰岛素的洗脱体积迁移。这与对照胰腺组织提取物的模式截然不同，对照胰腺组织提取物中的IRI峰位于胰岛素的洗脱体积处。我们得出结论，目前的转移性肝肿瘤不仅产生大量胰高血糖素，还产生其内部含有免疫胰岛素决定簇的异质性肽。
