Khurshid Sana, Afzal Madeeha, Khalid Ruqqya, Akhtar M Waheed, Qazi Mahmood Husain
Institute of Molecular Biology & Biotechnology, Defense Road, University of The Lahore, Lahore, Pakistan.
Institute of Molecular Biology & Biotechnology, Defense Road, University of The Lahore, Lahore, Pakistan.
Biologicals. 2017 Jul;48:109-113. doi: 10.1016/j.biologicals.2017.04.004. Epub 2017 May 15.
Tuberculosis is one of the top ten causes of deaths worldwide. The cause of tuberculosis is a bacterium, Mycobacterium tuberculosis, which has been surviving for centuries. Immunological tests based on detecting the presence of antibodies in the sera of active TB patients against various antigens of M. tuberculosis are useful for diagnosis of TB and offer simple, rapid and cost effective methods most suitable for poor and developing countries. Several recombinant antigens have been reported so far with varying sensitivity individually, yet none had shown sensitivity higher enough to be used in a commercial test. There is a trend of utilizing recombinant DNA technology to make polypeptide chain with two or more different antigenic regions, in order to increase the diagnostic efficiency. In this review, we have made an attempt to combine current studies on the usefulness of the multi-component Mycobacterium tuberculosis antigens in serological tests for the diagnosis of tuberculosis.
结核病是全球十大死因之一。结核病的病因是一种细菌——结核分枝杆菌，这种细菌已经存活了数百年。基于检测活动性肺结核患者血清中针对结核分枝杆菌各种抗原的抗体存在情况的免疫学检测，对于结核病的诊断很有用，并且提供了最适合贫困和发展中国家的简单、快速且经济高效的方法。到目前为止，已经报道了几种重组抗原，它们各自的敏感性各不相同，但没有一种显示出足够高的敏感性可用于商业检测。利用重组DNA技术制造具有两个或更多不同抗原区域的多肽链，以提高诊断效率，这是一种趋势。在这篇综述中，我们尝试结合目前关于多组分结核分枝杆菌抗原在血清学检测中诊断结核病的有用性的研究。
